UniProt Accession

 IDH_ECOLI; P08200 

Genbank Protein ID

 J02799; U00096; AP009048; AF017587 

Genbank Nucleotide ID

 AAA24006.1; AAC74220.1; BAA35958.1; AAC45887.1 

Protein Name

 Isocitrate dehydrogenase [NADP] 

Protein Synonyms/Alias

 IDH; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase 

Gene Name

 icd 

Gene Synonyms/Alias

 icdA; icdE; b1136; JW1122 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
4	****MESKVVVPAQG	acetylation	[1]
4	****MESKVVVPAQG	pupylation	[2]
12	VVVPAQGKKITLQNG	acetylation	[1]
13	VVPAQGKKITLQNGK	acetylation	[1]
20	KITLQNGKLNVPENP	acetylation	[1]
55	VVDAAVEKAYKGERK	acetylation	[1, 3]
58	AAVEKAYKGERKISW	acetylation	[3]
142	QGTPSPVKHPELTDM	acetylation	[1, 3, 4, 5]
166	IYAGIEWKADSADAE	acetylation	[1]
174	ADSADAEKVIKFLRE	acetylation	[1]
177	ADAEKVIKFLREEMG	acetylation	[1, 5]
186	LREEMGVKKIRFPEH	acetylation	[1]
199	EHCGIGIKPCSEEGT	acetylation	[1]
230	DSVTLVHKGNIMKFT	acetylation	[1, 5]
235	VHKGNIMKFTEGAFK	acetylation	[1, 3, 5]
242	KFTEGAFKDWGYQLA	acetylation	[1, 3, 5]
265	IDGGPWLKVKNPNTG	acetylation	[1, 5]
267	GGPWLKVKNPNTGKE	acetylation	[1]
273	VKNPNTGKEIVIKDV	acetylation	[1]
273	VKNPNTGKEIVIKDV	pupylation	[2]
350	PKYAGQDKVNPGSII	acetylation	[1, 3]
378	EAADLIVKGMEGAIN	acetylation	[1, 5]
387	MEGAINAKTVTYDFE	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [4] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [5] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

  

Sequence Annotation

 NP_BIND 339 345 NADP.
 METAL 307 307 Magnesium or manganese.
 BINDING 104 104 NADP.
 BINDING 113 113 Substrate.
 BINDING 115 115 Substrate.
 BINDING 119 119 Substrate.
 BINDING 129 129 Substrate.
 BINDING 153 153 Substrate.
 BINDING 352 352 NADP; via amide nitrogen and carbonyl
 BINDING 391 391 NADP.
 BINDING 395 395 NADP.
 MOD_RES 100 100 N6-succinyllysine.
 MOD_RES 113 113 Phosphoserine.
 MOD_RES 142 142 N6-acetyllysine.
 MOD_RES 242 242 N6-succinyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 416 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0022900; P:electron transport chain; IMP:EcoliWiki.
 GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 

Interpro

 IPR019818; IsoCit/isopropylmalate_DH_CS.
 IPR001804; Isocitrate/isopropylmalate_DH.
 IPR004439; Isocitrate_DH_NADP_dimer_prok.
 IPR024084; IsoPropMal-DH-like_dom. 

Pfam

 PF00180; Iso_dh 

SMART

  

PROSITE

 PS00470; IDH_IMDH 

PRINTS