UniProt Accession

 B3KNJ4_HUMAN; B3KNJ4 

Genbank Protein ID

 AC008532; AC008755; AK027595; CH471126 

Genbank Nucleotide ID

 BAG51356.1; EAW57460.1 

Protein Name

 SUMO-1 activating enzyme subunit 1, isoform CRA_a 

Protein Synonyms/Alias

 SUMO-activating enzyme subunit 1; cDNA FLJ14689 fis, clone NT2RP2005204, highly similar to Ubiquitin-like 1-activating enzyme E1A 

Gene Name

 SAE1 

Gene Synonyms/Alias

 hCG_20373 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
32	LWGLEAQKRLRASRV	ubiquitination	[1, 2, 3, 4, 5, 6]
45	RVLLVGLKGLGAEIA	ubiquitination	[2, 3, 4, 6]
53	GLGAEIAKNLILAGV	ubiquitination	[1, 2, 3, 4, 7]
61	NLILAGVKGLTMLDH	ubiquitination	[2, 4, 7]
109	LNPMVDVKVDTEDIE	ubiquitination	[2]
141	CSRDVIVKVDQICHK	ubiquitination	[4]
148	KVDQICHKNSIKFFT	ubiquitination	[4, 6]
152	ICHKNSIKFFTGDVF	ubiquitination	[4]
180	EFVEEKTKVAKVSQG	ubiquitination	[4]
183	EEKTKVAKVSQGVED	ubiquitination	[2, 4, 5, 6]
195	VEDGPDTKRAKLDSS	acetylation	[8]
195	VEDGPDTKRAKLDSS	ubiquitination	[2, 3, 4, 5, 6, 7]
198	GPDTKRAKLDSSETT	acetylation	[6]
198	GPDTKRAKLDSSETT	ubiquitination	[4, 6]
208	SSETTMVKKKVVFCP	ubiquitination	[2, 3, 4, 5, 6, 7]
209	SETTMVKKKVVFCPV	ubiquitination	[4]
210	ETTMVKKKVVFCPVK	ubiquitination	[4]
217	KVVFCPVKEALEVDW	ubiquitination	[4]
228	EVDWSSEKAKAALKR	acetylation	[9]
228	EVDWSSEKAKAALKR	ubiquitination	[2, 4, 5, 6]
230	DWSSEKAKAALKRTT	ubiquitination	[4, 6]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 299 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:HPA.
 GO:0008641; F:small protein activating enzyme activity; IEA:InterPro.
 GO:0006464; P:cellular protein modification process; IEA:InterPro. 

Interpro

 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR000011; UBQ/SUMO-activ_enz_E1-like. 

Pfam

 PF00899; ThiF 

SMART

  

PROSITE

  

PRINTS

 PR01849; UBIQUITINACT.