UniProt Accession

 GGCT_HUMAN; O75223; B2RDN0; B8ZZN4; B8ZZR8; Q9BS37 

Genbank Protein ID

 AK315608; AC005154; CH471073; CH471073; CH471073; BC000625; BC005356; BC013937; BC019243 

Genbank Nucleotide ID

 BAG37977.1; EAW93950.1; EAW93952.1; EAW93953.1; AAH00625.1; AAH05356.1; AAH13937.1; AAH19243.1 

Protein Name

 Gamma-glutamylcyclotransferase 

Protein Synonyms/Alias

 Cytochrome c-releasing factor 21 

Gene Name

 GGCT 

Gene Synonyms/Alias

 C7orf24; CRF21 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
118	KVATQEGKEITCRSY	ubiquitination	[1]
140	APPSPQYKKIICMGA	ubiquitination	[1, 2, 3, 4, 5]
141	PPSPQYKKIICMGAK	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and may play a significant role in glutathione homeostasis. Induces release of cytochrome c from mitochondria with resultant induction of apoptosis. 

Sequence Annotation

 REGION 19 22 Substrate binding (By similarity).
 ACT_SITE 98 98 Proton acceptor (By similarity).  

Keyword

 3D-structure; Acyltransferase; Alternative splicing; Complete proteome; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 188 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0003839; F:gamma-glutamylcyclotransferase activity; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0006750; P:glutathione biosynthetic process; TAS:Reactome.
 GO:0001836; P:release of cytochrome c from mitochondria; IMP:UniProtKB.
 GO:0006805; P:xenobiotic metabolic process; TAS:Reactome. 

Interpro

 IPR013024; Butirosin_synth_BtrG-like.
 IPR017939; G-Glutamylcylcotransferase. 

Pfam

  

SMART

  

PROSITE

  

PRINTS