UniProt Accession

 VAS1_HUMAN; Q15904; A6ZKI4 

Genbank Protein ID

 L44140; AK026519; AK289452; BX936347; BX936385; BX936385; BX936347; CH471172; BC000724; D16469 

Genbank Nucleotide ID

 BAF82141.1; CAI43213.3; CAI43213.3; CAI95782.2; CAI95782.2; EAW72715.1; AAH00724.1; BAA03938.1 

Protein Name

 V-type proton ATPase subunit S1 

Protein Synonyms/Alias

 V-ATPase subunit S1; Protein XAP-3; V-ATPase Ac45 subunit; V-ATPase S1 accessory protein; Vacuolar proton pump subunit S1 

Gene Name

 ATP6AP1 

Gene Synonyms/Alias

 ATP6IP1; ATP6S1; VATPS1; XAP3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
168	LATLRELKLNASLPA	ubiquitination	[1]
211	GQVLSTLKSEDVPYT	ubiquitination	[1, 2, 3]
380	YVSSLSKKGSLLVAR	ubiquitination	[2, 4, 5]
460	MDRFDDHKGPTISLT	ubiquitination	[2]

Reference

 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells (By similarity). 

Sequence Annotation

 CARBOHYD 170 170 N-linked (GlcNAc...) (Potential).
 CARBOHYD 261 261 N-linked (GlcNAc...).
 CARBOHYD 273 273 N-linked (GlcNAc...).
 CARBOHYD 296 296 N-linked (GlcNAc...) (Potential).
 CARBOHYD 303 303 N-linked (GlcNAc...) (Potential).
 CARBOHYD 350 350 N-linked (GlcNAc...) (Potential).
 CARBOHYD 357 357 N-linked (GlcNAc...) (Potential).  

Keyword

 ATP-binding; Complete proteome; Glycoprotein; Hydrogen ion transport; Ion transport; Membrane; Nucleotide-binding; Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport; Vacuole. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 470 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0016469; C:proton-transporting two-sector ATPase complex; TAS:ProtInc.
 GO:0033180; C:proton-transporting V-type ATPase, V1 domain; IEA:InterPro.
 GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046933; F:proton-transporting ATP synthase activity, rotational mechanism; IEA:InterPro.
 GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IEA:InterPro.
 GO:0005215; F:transporter activity; TAS:ProtInc.
 GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro.
 GO:0015992; P:proton transport; TAS:ProtInc. 

Interpro

 IPR008388; ATPase_V1-cplx_s1su.
 IPR024722; BIG/ATPase_V1_suS1. 

Pfam

 PF05827; ATP-synt_S1 

SMART

  

PROSITE

  

PRINTS