UniProt Accession

 TRM7_HUMAN; Q9UET6; B2RCJ0; O75670 

Genbank Protein ID

 AF063015; AJ005892; AK315138; AF196972; CH471224; BC023584 

Genbank Nucleotide ID

 AAC33734.1; CAA06749.1; BAG37587.1; EAW50782.1; AAH23584.1 

Protein Name

 Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase 

Protein Synonyms/Alias

 2'-O-ribose RNA methyltransferase TRM7 homolog; Protein ftsJ homolog 1 

Gene Name

 FTSJ1 

Gene Synonyms/Alias

 JM23 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
34	FKLLQLDKEFQLFQG	ubiquitination	[1, 2, 3, 4, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs (By similarity). 

Sequence Annotation

 ACT_SITE 156 156 Proton acceptor (By similarity).
 BINDING 53 53 S-adenosyl-L-methionine; via amide
 BINDING 55 55 S-adenosyl-L-methionine; via amide
 BINDING 75 75 S-adenosyl-L-methionine (By similarity).
 BINDING 91 91 S-adenosyl-L-methionine (By similarity).
 BINDING 116 116 S-adenosyl-L-methionine (By similarity).  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Mental retardation; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 329 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0001510; P:RNA methylation; IEA:InterPro.
 GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. 

Interpro

 IPR015507; rRNA-MeTfrase_E.
 IPR002877; rRNA_MeTrfase_FtsJ_dom. 

Pfam

 PF01728; FtsJ 

SMART

  

PROSITE

  

PRINTS