UniProt Accession

 PDK3_MOUSE; Q922H2; Q8BTX1 

Genbank Protein ID

 AK075879; AK075985; AK088478; BC008126 

Genbank Nucleotide ID

 BAC36024.1; BAC36097.1; BAC40379.1; AAH08126.1 

Protein Name

 [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial 

Protein Synonyms/Alias

 Pyruvate dehydrogenase kinase isoform 3 

Gene Name

 Pdk3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
278	KTLVTLGKEDLSIKI	acetylation	[1, 2]
278	KTLVTLGKEDLSIKI	succinylation	[2]

Reference

 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species (By similarity). 

Sequence Annotation

 DOMAIN 131 362 Histidine kinase.
 NP_BIND 247 254 ATP (By similarity).
 NP_BIND 306 307 ATP (By similarity).
 NP_BIND 323 328 ATP (By similarity).
 BINDING 287 287 ATP (By similarity).  

Keyword

 ATP-binding; Carbohydrate metabolism; Complete proteome; Glucose metabolism; Kinase; Mitochondrion; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 415 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
 GO:0004740; F:pyruvate dehydrogenase (acetyl-transferring) kinase activity; ISS:UniProtKB.
 GO:0071398; P:cellular response to fatty acid; ISS:UniProtKB.
 GO:0071333; P:cellular response to glucose stimulus; ISS:UniProtKB.
 GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW.
 GO:0097411; P:hypoxia-inducible factor-1alpha signaling pathway; ISS:UniProtKB.
 GO:0018105; P:peptidyl-serine phosphorylation; ISS:UniProtKB.
 GO:0035357; P:peroxisome proliferator activated receptor signaling pathway; ISS:UniProtKB.
 GO:0010510; P:regulation of acetyl-CoA biosynthetic process from pyruvate; ISS:UniProtKB.
 GO:0010906; P:regulation of glucose metabolic process; ISS:UniProtKB.
 GO:2000377; P:regulation of reactive oxygen species metabolic process; ISS:UniProtKB. 

Interpro

 IPR018955; BCDHK/PDK_N.
 IPR003594; HATPase_ATP-bd.
 IPR005467; Sig_transdc_His_kinase_core. 

Pfam

 PF10436; BCDHK_Adom3
 PF02518; HATPase_c 

SMART

 SM00387; HATPase_c 

PROSITE

 PS50109; HIS_KIN 

PRINTS