UniProt Accession

 GLSK_HUMAN; O94925; Q9UL05; Q9UL06; Q9UL07; Q9UN40 

Genbank Protein ID

 AF158555; AF097492; AF097493; AF097495; AB020645; AF223943; AF327434; BC038507; AF279697 

Genbank Nucleotide ID

 AAD47056.1; AAF00088.1; AAF00089.1; AAF00090.1; BAA74861.2; AAF33825.1; AAG47842.1; AAH38507.2; AAG17700.1 

Protein Name

 Glutaminase kidney isoform, mitochondrial 

Protein Synonyms/Alias

 GLS; K-glutaminase; L-glutamine amidohydrolase 

Gene Name

 GLS 

Gene Synonyms/Alias

 GLS1; KIAA0838 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
121	AFGNSEGKELVASGE	ubiquitination	[1]
158	QEKIPVHKFITALKS	ubiquitination	[1, 2]
164	HKFITALKSTGLRTS	ubiquitination	[2]
176	RTSDPRLKECMDMLR	ubiquitination	[3]
311	YVHRYVGKEPSGLRF	ubiquitination	[2]
568	LQQELALKETVWKKV	ubiquitination	[3]
574	LKETVWKKVSPESNE	ubiquitination	[3]

Reference

 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. 

Sequence Annotation

 REPEAT 585 614 ANK 1.
 REPEAT 619 648 ANK 2.
 BINDING 286 286 Substrate.
 BINDING 335 335 Substrate.
 BINDING 381 381 Substrate.
 BINDING 388 388 Substrate.
 BINDING 414 414 Substrate.
 BINDING 466 466 Substrate.
 BINDING 484 484 Substrate; via amide nitrogen.
 MOD_RES 311 311 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ANK repeat; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Mitochondrion; Polymorphism; Reference proteome; Repeat; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 669 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0004359; F:glutaminase activity; IDA:UniProtKB.
 GO:0007610; P:behavior; IEA:Compara.
 GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
 GO:0006537; P:glutamate biosynthetic process; IDA:UniProtKB.
 GO:0014047; P:glutamate secretion; TAS:Reactome.
 GO:0006543; P:glutamine catabolic process; IDA:UniProtKB.
 GO:0007269; P:neurotransmitter secretion; TAS:Reactome.
 GO:0051289; P:protein homotetramerization; IDA:UniProtKB.
 GO:0002087; P:regulation of respiratory gaseous exchange by neurological system process; IEA:Compara. 

Interpro

 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR012338; Beta-lactam/transpept-like.
 IPR015868; Glutaminase. 

Pfam

 PF12796; Ank_2
 PF04960; Glutaminase 

SMART

 SM00248; ANK 

PROSITE

 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 

PRINTS