CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014015
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrator complex subunit 11 
Protein Synonyms/Alias
 Int11; Cleavage and polyadenylation-specific factor 3-like protein; CPSF3-like protein; Protein related to CPSF subunits of 68 kDa; RC-68 
Gene Name
 CPSF3L 
Gene Synonyms/Alias
 INTS11; RC68 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
115RKIAVDKKGEANFFTubiquitination[1]
262FWERMNLKVPIYFSTubiquitination[1]
280EKANHYYKLFIPWTNubiquitination[2, 3]
289FIPWTNQKIRKTFVQubiquitination[2, 3]
418LLVHGEAKKMEFLKQubiquitination[1]
426KMEFLKQKIEQELRVubiquitination[4]
474QGLLPEAKKPRLLHGacetylation[5]
486LHGTLIMKDSNFRLVubiquitination[1, 2, 3]
500VSSEQALKELGLAEHubiquitination[1, 2, 3]
593FLTSLLKKGLPQAPSubiquitination[6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
 Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. 
Sequence Annotation
 MOTIF 68 73 HXHXDH motif.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Nucleus; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 600 AA 
Protein Sequence
MPEIRVTPLG AGQDVGRSCI LVSIAGKNVM LDCGMHMGFN DDRRFPDFSY ITQNGRLTDF 60
LDCVIISHFH LDHCGALPYF SEMVGYDGPI YMTHPTQAIC PILLEDYRKI AVDKKGEANF 120
FTSQMIKDCM KKVVAVHLHQ TVQVDDELEI KAYYAGHVLG AAMFQIKVGS ESVVYTGDYN 180
MTPDRHLGAA WIDKCRPNLL ITESTYATTI RDSKRCRERD FLKKVHETVE RGGKVLIPVF 240
ALGRAQELCI LLETFWERMN LKVPIYFSTG LTEKANHYYK LFIPWTNQKI RKTFVQRNMF 300
EFKHIKAFDR AFADNPGPMV VFATPGMLHA GQSLQIFRKW AGNEKNMVIM PGYCVQGTVG 360
HKILSGQRKL EMEGRQVLEV KMQVEYMSFS AHADAKGIMQ LVGQAEPESV LLVHGEAKKM 420
EFLKQKIEQE LRVNCYMPAN GETVTLPTSP SIPVGISLGL LKREMAQGLL PEAKKPRLLH 480
GTLIMKDSNF RLVSSEQALK ELGLAEHQLR FTCRVHLHDT RKEQETALRV YSHLKSVLKD 540
HCVQHLPDGS VTVESVLLQA AAPSEDPGTK VLLVSWTYQD EELGSFLTSL LKKGLPQAPS 600 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW. 
Interpro
 IPR001279; Beta-lactamas-like.
 IPR022712; Beta_Casp.
 IPR011108; RMMBL. 
Pfam
 PF10996; Beta-Casp
 PF00753; Lactamase_B
 PF07521; RMMBL 
SMART
 SM01027; Beta-Casp
 SM00849; Lactamase_B 
PROSITE
  
PRINTS