CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011929
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Splicing factor, suppressor of white-apricot homolog 
Protein Synonyms/Alias
 Splicing factor, arginine/serine-rich 8; Suppressor of white apricot protein homolog 
Gene Name
 SFSWAP 
Gene Synonyms/Alias
 SFRS8; SWAP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
315NRLEELMKPLKVVDPacetylation[1]
473YVARNGLKFETSVRAubiquitination[2]
935ITQDLMAKVRAMLAAacetylation[3, 4, 5]
944RAMLAASKNLQTSASubiquitination[2]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Plays a role as an alternative splicing regulator. Regulate its own expression at the level of RNA processing. Also regulates the splicing of fibronectin and CD45 genes. May act, at least in part, by interaction with other R/S-containing splicing factors. Represses the splicing of MAPT/Tau exon 10. 
Sequence Annotation
 REPEAT 211 253 SURP motif 1.
 REPEAT 459 499 SURP motif 2.
 MOD_RES 283 283 Phosphoserine.
 MOD_RES 315 315 N6-acetyllysine.
 MOD_RES 604 604 Phosphoserine.
 MOD_RES 642 642 Phosphothreonine.
 MOD_RES 909 909 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Repressor; RNA-binding; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 951 AA 
Protein Sequence
MYGASGGRAK PERKSGAKEE AGPGGAGGGG SRVELLVFGY ACKLFRDDER ALAQEQGQHL 60
IPWMGDHKIL IDRYDGRGHL HDLSEYDAEY STWNRDYQLS EEEARIEALC DEERYLALHT 120
DLLEEEARQE EEYKRLSEAL AEDGSYNAVG FTYGSDYYDP SEPTEEEEPS KQREKNEAEN 180
LEENEEPFVA PLGLSVPSDV ELPPTAKMHA IIERTASFVC RQGAQFEIML KAKQARNSQF 240
DFLRFDHYLN PYYKFIQKAM KEGRYTVLAE NKSDEKKKSG VSSDNEDDDD EEDGNYLHPS 300
LFASKKCNRL EELMKPLKVV DPDHPLAALV RKAQADSSTP TPHNADGAPV QPSQVEYTAD 360
STVAAMYYSY YMLPDGTYCL APPPPGIDVT TYYSTLPAGV TVSNSPGVTT TAPPPPGTTP 420
LPPPTTAETS SGATSTTTTT SALAPVAAII PPPPDVQPVI DKLAEYVARN GLKFETSVRA 480
KNDQRFEFLQ PWHQYNAYYE FKKQFFLQKE GGDSMQAVSA PEEAPTDSAP EKPSDAGEDG 540
APEDAAEVGA RAGSGGKKEA SSSKTVPDGK LVKASFAPIS FAIKAKENDL LPLEKNRVKL 600
DDDSDDDEES KEGQESSSSA ANTNPAVAPP CVVVEEKKPQ LTQEELEAKQ AKQKLEDRLA 660
AAAREKLAQA SKESKEKQLQ AERKRKAALF LQTLKNPLPE AEAGKIEESP FSVEESSTTP 720
CPLLTGGRPL PTLEVKPPDR PSSKSKDPPR EEEKEKKKKK HKKRSRTRSR SPKYHSSSKS 780
RSRSHSKAKH SLPSAYRTVR RSRSRSRSPR RRAHSPERRR EERSVPTAYR VSRSPGASRK 840
RTRSRSPHEK KKKRRSRSRT KSKARSQSVS PSKQAAPRPA APAAHSAHSA SVSPVESRGS 900
SQERSRGVSQ EKEAQISSAI VSSVQSKITQ DLMAKVRAML AASKNLQTSA S 951 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006376; P:mRNA splice site selection; TAS:ProtInc.
 GO:0048025; P:negative regulation of mRNA splicing, via spliceosome; ISS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR000061; Surp.
 IPR019147; SWAP_N_domain. 
Pfam
 PF09750; DRY_EERY
 PF01805; Surp 
SMART
 SM00648; SWAP 
PROSITE
 PS50128; SURP 
PRINTS