CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001692
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein SGT1 
Protein Synonyms/Alias
 hSGT1; Protein ecdysoneless homolog; Suppressor of GCR2 
Gene Name
 ECD 
Gene Synonyms/Alias
 SGT1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
68NLKYKPGKGGVPAHMubiquitination[1]
100YVIKQITKEFPELVAubiquitination[1]
153CIIPAPRKSGAESWLubiquitination[1]
201RIRGYPEKIQASLHRubiquitination[1]
250LRACRVFKTFLPETRubiquitination[1]
303RAHELGMKLAHGFEIubiquitination[1]
314GFEILCSKCSPHFSDubiquitination[1]
347LKKNDYFKGLIEGSAubiquitination[2, 3]
458TEVSESMKAFISKVSubiquitination[4]
463SMKAFISKVSTHKGAubiquitination[1, 2, 3, 5]
468ISKVSTHKGAELPREubiquitination[1, 6]
543KGTLDNLKSYMAQMDubiquitination[6]
560LAHTCISKSFTTRNQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
 Novel regulator of p53 stability and function. May also be a transcriptional activator required for the expression of glycolytic genes. 
Sequence Annotation
 MOD_RES 503 503 Phosphoserine.
 MOD_RES 505 505 Phosphoserine.
 MOD_RES 518 518 Phosphoserine.  
Keyword
 Activator; Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 644 AA 
Protein Sequence
MEETMKLATM EDTVEYCLFL IPDESRDSDK HKEILQKYIE RIITRFAPML VPYIWQNQPF 60
NLKYKPGKGG VPAHMFGVTK FGDNIEDEWF IVYVIKQITK EFPELVARIE DNDGEFLLIE 120
AADFLPKWLD PENSTNRVFF CHGELCIIPA PRKSGAESWL PTTPPTIPQA LNIITAHSEK 180
ILASESIRAA VNRRIRGYPE KIQASLHRAH CFLPAGIVAV LKQRPRLVAA AVQAFYLRDP 240
IDLRACRVFK TFLPETRIMT SVTFTKCLYA QLVQQRFVPD RRSGYRLPPP SDPQYRAHEL 300
GMKLAHGFEI LCSKCSPHFS DCKKSLVTAS PLWASFLESL KKNDYFKGLI EGSAQYRERL 360
EMAENYFQLS VDWPESSLAM SPGEEILTLL QTIPFDIEDL KKEAANLPPE DDDQWLDLSP 420
DQLDQLLQEA VGKKESESVS KEEKEQNYDL TEVSESMKAF ISKVSTHKGA ELPREPSEAP 480
ITFDADSFLN YFDKILGPRP NESDSDDLDD EDFECLDSDD DLDFETHEPG EEASLKGTLD 540
NLKSYMAQMD QELAHTCISK SFTTRNQVEP VSQTTDNNSD EEDSGTGESV MAPVDVDLNL 600
VSNILESYSS QAGLAGPASN LLQSMGVQLP DNTDHRPTSK PTKN 644 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0006110; P:regulation of glycolysis; TAS:ProtInc.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 
Interpro
 IPR010770; SGT1. 
Pfam
 PF07093; SGT1 
SMART
  
PROSITE
  
PRINTS