UniProt Accession

 UBE2A_MOUSE; Q9Z255 

Genbank Protein ID

 AF089812; AF383148; BC026053 

Genbank Nucleotide ID

 AAC64563.1; AAK62984.1; AAH26053.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 A 

Protein Synonyms/Alias

 RAD6 homolog A; HR6A; mHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A 

Gene Name

 Ube2a 

Gene Synonyms/Alias

 Rad6a 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
66	FTEEYPNKPPTVRFV	acetylation	[1]
66	FTEEYPNKPPTVRFV	ubiquitination	[2]

Reference

 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA (By similarity). 

Sequence Annotation

 ACT_SITE 88 88 Glycyl thioester intermediate (By  

Keyword

 ATP-binding; Complete proteome; DNA damage; DNA repair; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 152 AA 

Protein Sequence

Gene Ontology

 GO:0033503; C:HULC complex; ISS:UniProtKB.
 GO:0000790; C:nuclear chromatin; IDA:MGI.
 GO:0001741; C:XY body; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0033522; P:histone H2A ubiquitination; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IGI:MGI.
 GO:0060135; P:maternal process involved in female pregnancy; IMP:MGI.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0051865; P:protein autoubiquitination; IEA:Compara.
 GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
 GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
 GO:0009411; P:response to UV; IEA:Compara. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS