| Tag | Content |
|---|
| CPLM ID | CPLM-023717 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Mediator of RNA polymerase II transcription subunit 16 |
Protein Synonyms/Alias | Mediator complex subunit 16; Thyroid hormone receptor-associated protein 5; Thyroid hormone receptor-associated protein complex 95 kDa component; Trap95; Vitamin D3 receptor-interacting protein complex 92 kDa component; DRIP92 |
Gene Name | MED16 |
Gene Synonyms/Alias | DRIP92; THRAP5 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 149 | KLALHVEKSGASSFG | ubiquitination | [1] | | 158 | GASSFGEKFSRVKFS | ubiquitination | [1] | | 530 | STRILAMKASLCKLS | ubiquitination | [1] | | 827 | CGCVTMLKSPNRTTA | ubiquitination | [1] |
|
Reference | [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA. Mol Cell Proteomics. 2012 May;11(5):148-59. [ PMID: 22505724] |
Functional Description | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
Sequence Annotation | REPEAT 79 118 WD 1.
REPEAT 210 252 WD 2.
REPEAT 275 319 WD 3.
REPEAT 634 675 WD 4.
REPEAT 790 829 WD 5. |
Keyword | Activator; Alternative splicing; Complete proteome; Direct protein sequencing; Nucleus; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; WD repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 877 AA |
Protein Sequence | MCDLRRPAAG GMMDLAYVCE WEKWSKSTHC PSVPLACAWS CRNLIAFTMD LRSDDQDLTR 60
MIHILDTEHP WDLHSIPSEH HEAITCLEWD QSGSRLLSAD ADGQIKCWSM ADHLANSWES 120
SVGSLVEGDP IVALSWLHNG VKLALHVEKS GASSFGEKFS RVKFSPSLTL FGGKPMEGWI 180
AVTVSGLVTV SLLKPSGQVL TSTESLCRLR GRVALADIAF TGGGNIVVAT ADGSSASPVQ 240
FYKVCVSVVS EKCRIDTEIL PSLFMRCTTD LNRKDKFPAI THLKFLARDM SEQVLLCASS 300
QTSSIVECWS LRKEGLPVNN IFQQISPVVG DKQPTILKWR ILSATNDLDR VSAVALPKLP 360
ISLTNTDLKV ASDTQFYPGL GLALAFHDGS VHIVHRLSLQ TMAVFYSSAA PRPVDEPAMK 420
RPRTAGPAVH LKAMQLSWTS LALVGIDSHG KLSVLRLSPS MGHPLEVGLA LRHLLFLLEY 480
CMVTGYDWWD ILLHVQPSMV QSLVEKLHEE YTRQTAALQQ VLSTRILAMK ASLCKLSPCT 540
VTRVCDYHTK LFLIAISSTL KSLLRPHFLN TPDKSPGDRL TEICTKITDV DIDKVMINLK 600
TEEFVLDMNT LQALQQLLQW VGDFVLYLLA SLPNQGSLLR PGHSFLRDGT SLGMLRELMV 660
VIRIWGLLKP SCLPVYTATS DTQDSMSLLF RLLTKLWICF PSTGPCSVWV LLGWQPLPGR 720
DEGPASEPDE ALVDECCLLP SQLLIPSLDW LPASDGLVSR LQPKQPLRLQ FGRAPTLPGS 780
AATLQLDGLA RAPGQPKIDH LRRLHLGACP TEECKACTRC GCVTMLKSPN RTTAVKQWEQ 840
RWIKNCLCGG LWWRVPLSYP 860 |
Gene Ontology | GO:0016592; C:mediator complex; IDA:UniProtKB. GO:0004872; F:receptor activity; IDA:UniProtKB. GO:0046966; F:thyroid hormone receptor binding; IDA:UniProtKB. GO:0030375; F:thyroid hormone receptor coactivator activity; IDA:UniProtKB. GO:0042809; F:vitamin D receptor binding; NAS:UniProtKB. GO:0030521; P:androgen receptor signaling pathway; IDA:UniProtKB. GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB. GO:0006357; P:regulation of transcription from RNA polymerase II promoter; NAS:UniProtKB. GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |