UniProt Accession

 HNRPK_RAT; P61980; Q07244; Q15671; Q60577; Q922Y7; Q96J62 

Genbank Protein ID

 D17711 

Genbank Nucleotide ID

 BAA04566.1 

Protein Name

 Heterogeneous nuclear ribonucleoprotein K 

Protein Synonyms/Alias

 hnRNP K; dC stretch-binding protein; CSBP 

Gene Name

 Hnrnpk 

Gene Synonyms/Alias

 Hnrpk 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
405	LAGSIIGKGGQRIKQ	ubiquitination	[1]
461	SVKQYSGKFF*****	acetylation	[2]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single- stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non- coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest (By similarity). 

Sequence Annotation

 DOMAIN 42 104 KH 1.
 REPEAT 54 76 1-1.
 REPEAT 59 62 3-1.
 DOMAIN 144 209 KH 2.
 REPEAT 245 250 2-1.
 REPEAT 257 260 3-2.
 REPEAT 267 270 3-3.
 REPEAT 295 298 3-4.
 REPEAT 324 329 2-2.
 DOMAIN 387 451 KH 3.
 REPEAT 399 421 1-2.
 REPEAT 404 407 3-5.
 REGION 1 276 Necessary for interaction with DDX1 (By
 REGION 54 421 2 X 22 AA approximate repeats.
 REGION 59 407 5 X 4 AA repeats of G-X-G-G.
 REGION 209 337 Interaction with ZIK1 (By similarity).
 REGION 236 273 RNA-binding RGG-box.
 REGION 245 329 2 X 6 AA approximate repeats.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 116 116 Phosphoserine (By similarity).
 MOD_RES 214 214 Phosphoserine (By similarity).
 MOD_RES 216 216 Phosphoserine (By similarity).
 MOD_RES 284 284 Phosphoserine (By similarity).
 MOD_RES 296 296 Omega-N-methylated arginine (By
 MOD_RES 299 299 Omega-N-methylated arginine (By
 MOD_RES 379 379 Phosphoserine (By similarity).
 MOD_RES 380 380 Phosphotyrosine (By similarity).
 CROSSLNK 422 422 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Activator; Cell junction; Cell projection; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Glycoprotein; Isopeptide bond; Methylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Ribonucleoprotein; RNA-binding; Spliceosome; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 463 AA 

Protein Sequence

Gene Ontology

 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0042995; C:cell projection; IEA:UniProtKB-KW.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0002102; C:podosome; IEA:UniProtKB-SubCell.
 GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003697; F:single-stranded DNA binding; IDA:RGD.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR004087; KH_dom.
 IPR004088; KH_dom_type_1.
 IPR012987; ROK_N. 

Pfam

 PF00013; KH_1
 PF08067; ROKNT 

SMART

 SM00322; KH 

PROSITE

 PS50084; KH_TYPE_1 

PRINTS