CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039844
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha 
Protein Synonyms/Alias
  
Gene Name
 PIK3C2A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
199RAVDQVIKAVRKICSubiquitination[1]
233AVNLPRSKTADVTSLubiquitination[1, 2, 3]
294TDCAQSSKSVKEAWTubiquitination[2]
492ETLENDIKGKLLDILubiquitination[1, 3, 4]
494LENDIKGKLLDILHKubiquitination[3]
501KLLDILHKDSSLGLSubiquitination[1, 2]
509DSSLGLSKEDKAFLWubiquitination[1, 3]
518DKAFLWEKRYYCFKHubiquitination[2]
682LLGGVAEKVRQASGSubiquitination[2, 3]
710QSFFQKNKCRLPLKPubiquitination[2]
716NKCRLPLKPSLVAKEubiquitination[2]
782IMDKIWLKEGLDLRMubiquitination[1]
837PLAEWLRKYNPSEEEubiquitination[1]
891MFHIDFGKFLGHAQMubiquitination[3]
1034PILSFSPKTYSFRQDubiquitination[2, 3]
1292LKDFNLSKETVKWYQubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1306 AA 
Protein Sequence
MAAFCRSITK LKTKFPYTNH RTNPGYLLSP VTAQRNICGE NASVKVSIDI EGFQLPVTFT 60
CDVSSTVEII IMQALCWVHD DLNQVDVGSY VLKVCGQEEV LQNNHCLGSH EHIQNCRKWD 120
TEIRLQLLTF SAMCQNLART AEDDETPVDL NKHLYQIEKP CKEAMTRHPV EELLDSYHNQ 180
VELALQIENQ HRAVDQVIKA VRKICSALDG VETLAITESV KKLKRAVNLP RSKTADVTSL 240
FGGEDTSRSS TRGSLNPENP VQVSINQLTA AIYDLLRLHA NSGRSPTDCA QSSKSVKEAW 300
TTTEQLQFTI FAAHGISSNW VSNYEKYYLI CSLSHNGKDL FKPIQSKKVG TYKNFFYLIK 360
WDELIIFPIQ ISQLPLESVL HLTLFGILNQ SSGSSPDSNK QRKGPEALGK VSLPLFDFKR 420
FLTCGTKLLY LWTSSHTNSV PGTVTKKGYV MERIVLQVDF PSPAFDIIYT TPQVDRSIIQ 480
QHNLETLEND IKGKLLDILH KDSSLGLSKE DKAFLWEKRY YCFKHPNCLP KILASAPNWK 540
WVNLAKTYSL LHQWPALYPL IALELLDSKF ADQEVRSLAV TWIEAISDDE LTDLLPQFVQ 600
ALKYEIYLNS SLVQFLLSRA LGNIQIAHNL YWLLKDALHD VQFSTRYEHV LGALLSVGGK 660
RLREELLKQT KLVQLLGGVA EKVRQASGSA RQVVLQRSME RVQSFFQKNK CRLPLKPSLV 720
AKELNIKSCS FFSSNAVPLK VTMVNADPMG EEINVMFKVG EDLRQDMLAL QMIKIMDKIW 780
LKEGLDLRMV IFKCLSTGRD RGMVELVPAS DTLRKIQVEY GVTGSFKDKP LAEWLRKYNP 840
SEEEYEKASE NFIYSCAGCC VATYVLGICD RHNDNIMLRS TGHMFHIDFG KFLGHAQMFG 900
SFKRDRAPFV LTSDMAYVIN GGEKPTIRFQ LFVDLCCQAY NLIRKQTNLF LNLLSLMIPS 960
GLPELTSIQD LKYVRDALQP QTTDAEATIF FTRLIESSLG SIATKFNFFI HNLAQLRFSG 1020
LPSNDEPILS FSPKTYSFRQ DGRIKEVSVF TYHKKYNPDK HYIYVVRILR EGQIEPSFVF 1080
RTFDEFQELH NKLSIIFPLW KLPGFPNRMV LGRTHIKDVA AKRKIELNSY LQSLMNASTD 1140
VAECDLVCTF FHPLLRDEKA EGIARSADAG SFSPTPGQIG GAVKLSISYR NGTLFIMVMH 1200
IKDLVTEDGA DPNPYVKTYL LPDNHKTSKR KTKISRKTRN PTFNEMLVYS GYSKETLRQR 1260
ELQLSVLSAE SLRENFFLGG VTLPLKDFNL SKETVKWYQL TAATYL 1306 
Gene Ontology
 GO:0016303; F:1-phosphatidylinositol-3-kinase activity; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0035091; F:phosphatidylinositol binding; IEA:InterPro.
 GO:0048015; P:phosphatidylinositol-mediated signaling; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR011009; Kinase-like_dom.
 IPR001683; Phox.
 IPR000403; PI3/4_kinase_cat_dom.
 IPR018936; PI3/4_kinase_CS.
 IPR002420; PI3K_C2_dom.
 IPR000341; PI3K_Ras-bd_dom.
 IPR015433; PI_Kinase.
 IPR001263; PInositide-3_kin_accessory_dom. 
Pfam
 PF00168; C2
 PF00454; PI3_PI4_kinase
 PF00792; PI3K_C2
 PF00794; PI3K_rbd
 PF00613; PI3Ka
 PF00787; PX 
SMART
 SM00239; C2
 SM00142; PI3K_C2
 SM00144; PI3K_rbd
 SM00145; PI3Ka
 SM00146; PI3Kc
 SM00312; PX 
PROSITE
 PS50004; C2
 PS00915; PI3_4_KINASE_1
 PS00916; PI3_4_KINASE_2
 PS50290; PI3_4_KINASE_3
 PS51547; PI3K_C2
 PS51546; PI3K_RBD
 PS51545; PIK_HELICAL
 PS50195; PX 
PRINTS