CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002988
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamate 5-kinase 
Protein Synonyms/Alias
 Gamma-glutamyl kinase; GK 
Gene Name
 proB 
Gene Synonyms/Alias
 b0242; JW0232 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
72LPATIASKQLLAAVGacetylation[1]
172LLLLTDQKGLYTADPacetylation[1]
189NPQAELIKDVYGIDDacetylation[1]
217GTGGMSTKLQAADVAacetylation[1]
298RGSSLLPKGIKSVTGacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline. 
Sequence Annotation
 DOMAIN 275 353 PUA.
 NP_BIND 169 170 ATP (Probable).
 NP_BIND 211 217 ATP (Probable).
 BINDING 10 10 ATP (Probable).
 BINDING 50 50 Substrate.
 BINDING 137 137 Substrate.
 BINDING 149 149 Substrate; via amide nitrogen.  
Keyword
 3D-structure; Amino-acid biosynthesis; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Nucleotide-binding; Proline biosynthesis; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 367 AA 
Protein Sequence
MSDSQTLVVK LGTSVLTGGS RRLNRAHIVE LVRQCAQLHA AGHRIVIVTS GAIAAGREHL 60
GYPELPATIA SKQLLAAVGQ SRLIQLWEQL FSIYGIHVGQ MLLTRADMED RERFLNARDT 120
LRALLDNNIV PVINENDAVA TAEIKVGDND NLSALAAILA GADKLLLLTD QKGLYTADPR 180
SNPQAELIKD VYGIDDALRA IAGDSVSGLG TGGMSTKLQA ADVACRAGID TIIAAGSKPG 240
VIGDVMEGIS VGTLFHAQAT PLENRKRWIF GAPPAGEITV DEGATAAILE RGSSLLPKGI 300
KSVTGNFSRG EVIRICNLEG RDIAHGVSRY NSDALRRIAG HHSQEIDAIL GYEYGPVAVH 360
RDDMITR 367 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004349; F:glutamate 5-kinase activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IDA:EcoCyc.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0055129; P:L-proline biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006561; P:proline biosynthetic process; IMP:EcoCyc. 
Interpro
 IPR001048; Asp/Glu/Uridylate_kinase.
 IPR001057; Glu/AcGlu_kinase.
 IPR011529; Glu_5kinase.
 IPR005715; Glu_5kinase/COase_Synthase.
 IPR019797; Glutamate_5-kinase_CS.
 IPR002478; PUA.
 IPR015947; PUA-like_domain. 
Pfam
 PF00696; AA_kinase
 PF01472; PUA 
SMART
 SM00359; PUA 
PROSITE
 PS00902; GLUTAMATE_5_KINASE
 PS50890; PUA 
PRINTS
 PR00474; GLU5KINASE.