CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016174
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Retrotransposon-derived protein PEG10 
Protein Synonyms/Alias
 Embryonal carcinoma differentiation-regulated protein; Mammalian retrotransposon-derived protein 2; Myelin expression factor 3-like protein 1; MEF3-like protein 1; Paternally expressed gene 10 protein; Retrotransposon gag domain-containing protein 3; Retrotransposon-derived gag-like polyprotein; Ty3/Gypsy-like protein 
Gene Name
 PEG10 
Gene Synonyms/Alias
 EDR; KIAA1051; MAR2; MART2; MEF3L1; RGAG3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22NLREKVMKQSEENNNubiquitination[1, 2, 3]
36NLQSQVQKLTEENTTubiquitination[1, 3]
136AARWASAKLERSHYLubiquitination[1, 2]
155PAFMMEMKHVFEDPQacetylation[4]
567DFVARNVKDGLITPTubiquitination[1, 2, 3, 5, 6, 7, 8]
586GAQVLQVKRGWKLQVubiquitination[1, 2, 3, 6, 7, 8]
590LQVKRGWKLQVSYDCubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [5] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter (By similarity). 
Sequence Annotation
 ZN_FING 293 310 CCHC-type.
 REGION 76 275 Necessary for interaction with ALK1.  
Keyword
 Apoptosis; Coiled coil; Complete proteome; Cytoplasm; Differentiation; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Ribosomal frameshifting; Transposable element; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 708 AA 
Protein Sequence
MTERRRDELS EEINNLREKV MKQSEENNNL QSQVQKLTEE NTTLREQVEP TPEDEDDDIE 60
LRGAAAAAAP PPPIEEECPE DLPEKFDGNP DMLAPFMAQC QIFMEKSTRD FSVDRVRVCF 120
VTSMMTGRAA RWASAKLERS HYLMHNYPAF MMEMKHVFED PQRREVAKRK IRRLRQGMGS 180
VIDYSNAFQM IAQDLDWNEP ALIDQYHEGL SDHIQEELSH LEVAKSLSAL IGQCIHIERR 240
LARAAAARKP RSPPRALVLP HIASHHQVDP TEPVGGARMR LTQEEKERRR KLNLCLYCGT 300
GGHYADNCPA KASKSSPAGK LPGPAVEGPS ATGPEIIRSP QDDASSPHLQ VMLQIHLPGR 360
HTLFVRAMID SGASGNFIDH EYVAQNGIPL RIKDWPILVE AIDGRPIASG PVVHETHDLI 420
VDLGDHREVL SFDVTQSPFF PVVLGVRWLS THDPNITWST RSIVFDSEYC RYHCRMYSPI 480
PPSLPPPAPQ PPLYYPVDGY RVYQPVRYYY VQNVYTPVDE HVYPDHRLVD PHIEMIPGAH 540
SIPSGHVYSL SEPEMAALRD FVARNVKDGL ITPTIAPNGA QVLQVKRGWK LQVSYDCRAP 600
NNFTIQNQYP RLSIPNLEDQ AHLATYTEFV PQIPGYQTYP TYAAYPTYPV GFAWYPVGRD 660
GQGRSLYVPV MITWNPHWYR QPPVPQYPPP QPPPPPPPPP PPPSYSTL 708 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HGNC.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IDA:HGNC.
 GO:0001890; P:placenta development; IEA:Compara. 
Interpro
 IPR021109; Peptidase_aspartic.
 IPR005162; Retrotrans_gag.
 IPR013242; RVP_2.
 IPR001878; Znf_CCHC. 
Pfam
 PF03732; Retrotrans_gag
 PF08284; RVP_2 
SMART
 SM00343; ZnF_C2HC 
PROSITE
 PS50158; ZF_CCHC 
PRINTS