CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014809
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Switch-associated protein 70 
Protein Synonyms/Alias
 SWAP-70 
Gene Name
 Swap70 
Gene Synonyms/Alias
 Kiaa0640 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
145EIEYLLKKLTEAMGGubiquitination[1]
272LPDKDGKKCLFLIKCacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity). 
Sequence Annotation
 DOMAIN 210 306 PH.  
Keyword
 Cell membrane; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Membrane; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 585 AA 
Protein Sequence
MRGLKDELLK AIWHAFTALD LDRSGKVSKS QLKVLSHNLC TVLKVPHDPV ALEEHFRDDD 60
EGPVSNQGYM PYLNKFILEK VQDNFDKIEF NRMCWTLCVK KNLTKSPLLI TEDDAFKVWV 120
IFNFLSEDKY PLIIVPEEIE YLLKKLTEAM GGGWQQEQFE HYKINFDDNK DGLSAWELIE 180
LIGNGQFSKG MDRQTVSMAI NEVFNELILD VLKQGYMMKK GHKRKNWTER WFVLKPNIIS 240
YYVSEDLKDK KGDILLDENC CVESLPDKDG KKCLFLIKCF DKTFEISASD KKKKQEWIQA 300
IYSTIHLLKL GSPPPHKEAR QRRKELRRKL LAEQEELERQ MKELQAANEN KQQELESVRK 360
KLEEAASRAA DEEKKRLQTQ VELQTRFSTE LEREKLIRQQ MEEQVAQKSS ELEQYLQRVR 420
ELEDMYLKLQ EALEDERQAR QDEETVRKLQ ARLLEEESSK RAELEKWHLE QQQAIQTTEA 480
EKQELEQQRV MKEQALQEAM AQLEQLELER KQALEQYEGV KKKLEMATHM TKSWKDKVAH 540
HEGLIRLIEP GSKNPHLITN WGPAAFTQAE LEEREKSWKE KKTTE 585 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IDA:MGI.
 GO:0003677; F:DNA binding; IDA:MGI.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0045190; P:isotype switching; IDA:MGI. 
Interpro
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF00169; PH 
SMART
 SM00233; PH 
PROSITE
 PS50003; PH_DOMAIN 
PRINTS