CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022885
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 STE20/SPS1-related proline-alanine-rich protein kinase 
Protein Synonyms/Alias
 Ste-20-related kinase; DCHT; Serine/threonine-protein kinase 39 
Gene Name
 STK39 
Gene Synonyms/Alias
 SPAK 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
83VVQAALCKPRQERVAubiquitination[1]
312KYGKSFRKLLSLCLQubiquitination[1]
324CLQKDPSKRPTAAELubiquitination[1]
349NREYLIEKLLTRTPDacetylation[2]
349NREYLIEKLLTRTPDubiquitination[1, 3, 4]
512QKIVDDPKALKTLTFacetylation[5]
515VDDPKALKTLTFKLAubiquitination[1]
520ALKTLTFKLASGCDGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May act as a mediator of stress-activated signals. 
Sequence Annotation
 DOMAIN 63 337 Protein kinase.
 NP_BIND 69 77 ATP (By similarity).
 MOTIF 360 366 Nuclear localization signal (Potential).
 MOTIF 387 391 Caspase cleavage related site.
 ACT_SITE 192 192 Proton acceptor (By similarity).
 BINDING 92 92 ATP (By similarity).
 MOD_RES 309 309 Phosphoserine; by PKC/PRKCQ.
 MOD_RES 349 349 N6-acetyllysine.
 MOD_RES 354 354 Phosphothreonine (By similarity).
 MOD_RES 370 370 Phosphoserine.
 MOD_RES 371 371 Phosphoserine.
 MOD_RES 385 385 Phosphoserine.  
Keyword
 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 545 AA 
Protein Sequence
MAEPSGSPVH VQLPQQAAPV TAAAAAAPAA ATAAPAPAAP AAPAPAPAPA AQAVGWPICR 60
DAYELQEVIG SGATAVVQAA LCKPRQERVA IKRINLEKCQ TSMDELLKEI QAMSQCSHPN 120
VVTYYTSFVV KDELWLVMKL LSGGSMLDII KYIVNRGEHK NGVLEEAIIA TILKEVLEGL 180
DYLHRNGQIH RDLKAGNILL GEDGSVQIAD FGVSAFLATG GDVTRNKVRK TFVGTPCWMA 240
PEVMEQVRGY DFKADMWSFG ITAIELATGA APYHKYPPMK VLMLTLQNDP PTLETGVEDK 300
EMMKKYGKSF RKLLSLCLQK DPSKRPTAAE LLKCKFFQKA KNREYLIEKL LTRTPDIAQR 360
AKKVRRVPGS SGHLHKTEDG DWEWSDDEMD EKSEEGKAAF SQEKSRRVKE ENPEIAVSAS 420
TIPEQIQSLS VHDSQGPPNA NEDYREASSC AVNLVLRLRN SRKELNDIRF EFTPGRDTAD 480
GVSQELFSAG LVDGHDVVIV AANLQKIVDD PKALKTLTFK LASGCDGSEI PDEVKLIGFA 540
QLSVS 545 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IEA:Compara.
 GO:0016323; C:basolateral plasma membrane; IEA:Compara.
 GO:0005737; C:cytoplasm; NAS:UniProtKB.
 GO:0005856; C:cytoskeleton; IEA:Compara.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0004702; F:receptor signaling protein serine/threonine kinase activity; NAS:UniProtKB.
 GO:0043268; P:positive regulation of potassium ion transport; IEA:Compara.
 GO:0050727; P:regulation of inflammatory response; IEA:Compara.
 GO:0006950; P:response to stress; NAS:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR024678; Kinase_OSR1/WNK_CCT.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom. 
Pfam
 PF12202; OSR1_C
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS