CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017788
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hydroxyacid-oxoacid transhydrogenase, mitochondrial 
Protein Synonyms/Alias
 HOT; Alcohol dehydrogenase iron-containing protein 1; ADHFe1 
Gene Name
 Adhfe1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
60RYGAGVTKEVGMDLQacetylation[1]
433DDIPSLVKGTLPQERacetylation[2]
443LPQERVTKLAPRAQSacetylation[2, 3]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 
Functional Description
 Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2- hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity). 
Sequence Annotation
  
Keyword
 Alternative splicing; Complete proteome; Mitochondrion; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 465 AA 
Protein Sequence
MAAAARARVT HLLRHLQSTA CQCPTHSHTY SQAPGPSGKT ADYAFEMAVS NIRYGAGVTK 60
EVGMDLQNMG AKNVCLMTDK NLSQLPPVQI VMDSLSKNGI SFQVYDDVRV EPTDGSFMDA 120
IEFAKKGAFD AYVAVGGGST MDTCKAANLY ASSPHSEFLD YVNAPIGKGK PVTVPLKPLI 180
AVPTTSGTGS ETTGVAIFDY EHLKVKTGIA SRAIKPTLGL VDPLHTLHMP CQVVANSGFD 240
VLCHALESYT AIPYSMRSPC PSNPIQRPAY QGSNPISDIW AVHALQIVAK YLKRAVRNPD 300
DLEARSKMHL ASAFAGIGFG NAGVHLCHGM SYPISGLVKT YKAKEYNVDH PLVPHGLSVV 360
LTSPAVFTFT AQMFPERHLE TAGILGANIR TARIQDAGLV LADALRKFLF DLNVDDGLAA 420
LGYSKDDIPS LVKGTLPQER VTKLAPRAQS EEDLSALFEA SMKLY 465 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0047988; F:hydroxyacid-oxoacid transhydrogenase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0015993; P:molecular hydrogen transport; ISS:UniProtKB. 
Interpro
 IPR001670; ADH_Fe. 
Pfam
 PF00465; Fe-ADH 
SMART
  
PROSITE
 PS00913; ADH_IRON_1
 PS00060; ADH_IRON_2 
PRINTS