UniProt Accession

 LASP1_HUMAN; Q14847; Q96ED2; Q96IG0 

Genbank Protein ID

 X82456; BC007560; BC012460 

Genbank Nucleotide ID

 CAA57833.1; AAH07560.1; AAH12460.1 

Protein Name

 LIM and SH3 domain protein 1 

Protein Synonyms/Alias

 LASP-1; Metastatic lymph node gene 50 protein; MLN 50 

Gene Name

 LASP1 

Gene Synonyms/Alias

 MLN50 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
10	PNCARCGKIVYPTEK	ubiquitination	[1]
17	KIVYPTEKVNCLDKF	ubiquitination	[1]
23	EKVNCLDKFWHKACF	ubiquitination	[1, 2]
42	CKMTLNMKNYKGYEK	ubiquitination	[1]
59	YCNAHYPKQSFTMVA	ubiquitination	[1]
75	TPENLRLKQQSELQS	ubiquitination	[1]
96	EFEKNKGKGFSVVAD	ubiquitination	[1]
121	QDQISNIKYHEEFEK	ubiquitination	[1, 2]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity). 

Sequence Annotation

 DOMAIN 5 56 LIM zinc-binding.
 REPEAT 61 95 Nebulin 1.
 REPEAT 97 131 Nebulin 2.
 DOMAIN 202 261 SH3.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 42 42 N6-acetyllysine.
 MOD_RES 68 68 Phosphothreonine.
 MOD_RES 104 104 Phosphothreonine.
 MOD_RES 118 118 Phosphoserine.
 MOD_RES 146 146 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Actin-binding; Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Ion transport; LIM domain; Metal-binding; Phosphoprotein; Reference proteome; Repeat; SH3 domain; Transport; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 261 AA 

Protein Sequence

Gene Ontology

 GO:0030864; C:cortical actin cytoskeleton; ISS:UniProtKB.
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0015075; F:ion transmembrane transporter activity; ISS:UniProtKB.
 GO:0005070; F:SH3/SH2 adaptor activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 

Interpro

 IPR000900; Nebulin_35r-motif.
 IPR001452; SH3_domain.
 IPR001781; Znf_LIM. 

Pfam

 PF00412; LIM
 PF00880; Nebulin
 PF00018; SH3_1 

SMART

 SM00132; LIM
 SM00227; NEBU
 SM00326; SH3 

PROSITE

 PS00478; LIM_DOMAIN_1
 PS50023; LIM_DOMAIN_2
 PS51216; NEBULIN
 PS50002; SH3 

PRINTS

 PR00452; SH3DOMAIN.