CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022169
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ufm1-specific protease 2 
Protein Synonyms/Alias
 UfSP2 
Gene Name
 UFSP2 
Gene Synonyms/Alias
 C4orf20 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
97FEPEEDIKRKFMRKKubiquitination[1, 2]
99PEEDIKRKFMRKKDKubiquitination[2]
244PHDRPYFKRSNAYHFubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of UFM1, an ubiquitin-like modifier protein bound to a number of target proteins. Does not hydrolyze SUMO1 or ISG15 ubiquitin-like proteins (By similarity). 
Sequence Annotation
 ACT_SITE 302 302 By similarity.
 ACT_SITE 426 426 By similarity.
 ACT_SITE 428 428 By similarity.  
Keyword
 Complete proteome; Cytoplasm; Endoplasmic reticulum; Hydrolase; Nucleus; Polymorphism; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 469 AA 
Protein Sequence
MVISESMDIL FRIRGGLDLA FQLATPNEIF LKKALKHVLS DLSTKLSSNA LVFRICHSSV 60
YIWPSSDINT IPGELTDASA CKNILRFIQF EPEEDIKRKF MRKKDKKLSD MHQIVNIDLM 120
LEMSTSLAAV TPIIERESGG HHYVNMTLPV DAVISVAPEE TWGKVRKLLV DAIHNQLTDM 180
EKCILKYMKG TSIVVPEPLH FLLPGKKNLV TISYPSGIPD GQLQAYRKEL HDLFNLPHDR 240
PYFKRSNAYH FPDEPYKDGY IRNPHTYLNP PNMETGMIYV VQGIYGYHHY MQDRIDDNGW 300
GCAYRSLQTI CSWFKHQGYT ERSIPTHREI QQALVDAGDK PATFVGSRQW IGSIEVQLVL 360
NQLIGITSKI LFVSQGSEIA SQGRELANHF QSEGTPVMIG GGVLAHTILG VAWNEITGQI 420
KFLILDPHYT GAEDLQVILE KGWCGWKGPD FWNKDAYYNL CLPQRPNMI 469 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0019783; F:small conjugating protein-specific protease activity; ISS:UniProtKB.
 GO:0016790; F:thiolester hydrolase activity; IEA:Compara.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR012462; Peptidase_C78_UfSP1/2. 
Pfam
 PF07910; Peptidase_C78 
SMART
  
PROSITE
  
PRINTS