UniProt Accession

 PRS10_YEAST; P53549; D6W2W0; Q08718 

Genbank Protein ID

 U43720; U93262; Z75167; BK006948 

Genbank Nucleotide ID

 AAA85134.1; AAB51594.1; CAA99481.1; DAA11026.1 

Protein Name

 26S protease subunit RPT4 

Protein Synonyms/Alias

 26S protease subunit SUG2; Proteasomal cap subunit 

Gene Name

 RPT4 

Gene Synonyms/Alias

 CRL13; PCS1; SUG2; YOR259C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
75	RRYDDQLKQRRQNIR	ubiquitination	[1]
362	AGRLEIFKIHTAKVK	acetylation	[2]
427	RKVAEVKKLEGTIEY	ubiquitination	[3]
436	EGTIEYQKL******	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). 

Sequence Annotation

 NP_BIND 222 229 ATP (Potential).
 MOD_RES 2 2 N-acetylserine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Nucleotide-binding; Proteasome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 437 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0008540; C:proteasome regulatory particle, base subcomplex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; ISS:SGD.
 GO:0019904; F:protein domain specific binding; IDA:SGD.
 GO:0030433; P:ER-associated protein catabolic process; IMP:SGD.
 GO:0006289; P:nucleotide-excision repair; IGI:SGD.
 GO:0045899; P:positive regulation of RNA polymerase II transcriptional preinitiation complex assembly; IMP:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:SGD.
 GO:0070682; P:proteasome regulatory particle assembly; IMP:SGD. 

Interpro

 IPR005937; 26S_Psome_P45.
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00004; AAA 

SMART

 SM00382; AAA 

PROSITE

 PS00674; AAA 

PRINTS