CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001579
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 
Protein Synonyms/Alias
 PAPS synthase 2; PAPSS 2; Sulfurylase kinase 2; SK 2; SK2; Sulfate adenylyltransferase; ATP-sulfurylase; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; 3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylylsulfate 3'-phosphotransferase 
Gene Name
 PAPSS2 
Gene Synonyms/Alias
 ATPSK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
173RARAGEIKGFTGIDSubiquitination[1]
184GIDSDYEKPETPERVubiquitination[1]
599PDGFMAPKAWKVLTDubiquitination[2]
602FMAPKAWKVLTDYYRubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. May have a important role in skeletogenesis during postnatal growth (By similarity). 
Sequence Annotation
 NP_BIND 49 56 ATP (Potential).
 REGION 1 ?210 Adenylyl-sulfate kinase.
 REGION ?211 614 Sulfate adenylyltransferase.
 MOTIF 511 515 PP-motif.
 ACT_SITE 123 123 Phosphoserine intermediate (By  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Disease mutation; Dwarfism; Kinase; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 614 AA 
Protein Sequence
MSGIKKQKTE NQQKSTNVVY QAHHVSRNKR GQVVGTRGGF RGCTVWLTGL SGAGKTTISF 60
ALEEYLVSHA IPCYSLDGDN VRHGLNRNLG FSPGDREENI RRIAEVAKLF ADAGLVCITS 120
FISPFAKDRE NARKIHESAG LPFFEIFVDA PLNICESRDV KGLYKRARAG EIKGFTGIDS 180
DYEKPETPER VLKTNLSTVS DCVHQVVELL QEQNIVPYTI IKDIHELFVP ENKLDHVRAE 240
AETLPSLSIT KLDLQWVQVL SEGWATPLKG FMREKEYLQV MHFDTLLDDG VINMSIPIVL 300
PVSAEDKTRL EGCSKFVLAH GGRRVAILRD AEFYEHRKEE RCSRVWGTTC TKHPHIKMVM 360
ESGDWLVGGD LQVLEKIRWN DGLDQYRLTP LELKQKCKEM NADAVFAFQL RNPVHNGHAL 420
LMQDTRRRLL ERGYKHPVLL LHPLGGWTKD DDVPLDWRMK QHAAVLEEGV LDPKSTIVAI 480
FPSPMLYAGP TEVQWHCRSR MIAGANFYIV GRDPAGMPHP ETKKDLYEPT HGGKVLSMAP 540
GLTSVEIIPF RVAAYNKAKK AMDFYDPARH NEFDFISGTR MRKLAREGEN PPDGFMAPKA 600
WKVLTDYYRS LEKN 614 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0004020; F:adenylylsulfate kinase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004781; F:sulfate adenylyltransferase (ATP) activity; ISS:UniProtKB.
 GO:0050428; P:3'-phosphoadenosine 5'-phosphosulfate biosynthetic process; TAS:Reactome.
 GO:0007596; P:blood coagulation; IEA:Compara.
 GO:0060348; P:bone development; IEA:Compara.
 GO:0001501; P:skeletal system development; TAS:ProtInc.
 GO:0000103; P:sulfate assimilation; IEA:UniProtKB-UniPathway.
 GO:0006805; P:xenobiotic metabolic process; TAS:Reactome. 
Interpro
 IPR002891; APS_kinase.
 IPR025980; ATP-Sase_PUA-like_dom.
 IPR027417; P-loop_NTPase.
 IPR015947; PUA-like_domain.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR024951; Sulfurylase_cat_dom.
 IPR002650; Sulphate_adenylyltransferase. 
Pfam
 PF01583; APS_kinase
 PF01747; ATP-sulfurylase
 PF14306; PUA_2 
SMART
  
PROSITE
  
PRINTS