CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020416
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 UBX domain-containing protein 6 
Protein Synonyms/Alias
 UBX domain-containing protein 1 
Gene Name
 UBXN6 
Gene Synonyms/Alias
 UBXD1; UBXDC2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
3*****MKKFFQEFKAubiquitination[1]
24AGPGQKLKESVGEKAubiquitination[2]
85TIRNQVRKELQAEATubiquitination[2, 3]
166MKIYTFNKDQDRVKLubiquitination[2, 4]
172NKDQDRVKLGVDTIAubiquitination[2, 3]
180LGVDTIAKYLDNIHLubiquitination[2, 3]
193HLHPEEEKYRKIKLQubiquitination[2]
196PEEEKYRKIKLQNKVubiquitination[2]
198EEKYRKIKLQNKVFQubiquitination[2]
202RKIKLQNKVFQERINubiquitination[2, 3]
257PQSLERHKEQLLAAEubiquitination[2, 4]
301NLTAEEIKREQRLRSubiquitination[2, 3]
333EEQRGLRKYNYTLLRubiquitination[2]
430AEPDSILKPELLSAIubiquitination[2, 3]
439ELLSAIEKLL*****ubiquitination[2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Acts in a complex with VCP and cooperates with USP7 in promoting MDM2 deubiquitination and stabilization. MDM2 stabilization leads to MDM2-dependent TP53 degradation. 
Sequence Annotation
 DOMAIN 175 244 PUB.
 DOMAIN 332 408 UBX.
 MOD_RES 96 96 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 441 AA 
Protein Sequence
MKKFFQEFKA DIKFKSAGPG QKLKESVGEK AHKEKPNQPA PRPPRQGPTN EAQMAAAAAL 60
ARLEQKQSRA WGPTSQDTIR NQVRKELQAE ATVSGSPEAP GTNVVSEPRE EGSAHLAVPG 120
VYFTCPLTGA TLRKDQRDAC IKEAILLHFS TDPVAASIMK IYTFNKDQDR VKLGVDTIAK 180
YLDNIHLHPE EEKYRKIKLQ NKVFQERINC LEGTHEFFEA IGFQKVLLPA QDQEDPEEFY 240
VLSETTLAQP QSLERHKEQL LAAEPVRAKL DRQRRVFQPS PLASQFELPG DFFNLTAEEI 300
KREQRLRSEA VERLSVLRTK AMREKEEQRG LRKYNYTLLR VRLPDGCLLQ GTFYARERLG 360
AVYGFVREAL QSDWLPFELL ASGGQKLSED ENLALNECGL VPSALLTFSW DMAVLEDIKA 420
AGAEPDSILK PELLSAIEKL L 441 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IDA:HPA. 
Interpro
 IPR018997; PUB_domain.
 IPR006567; PUG-dom.
 IPR001012; UBX. 
Pfam
 PF09409; PUB
 PF00789; UBX 
SMART
 SM00580; PUG
 SM00166; UBX 
PROSITE
 PS50033; UBX 
PRINTS