UniProt Accession

 CSK2B_HUMAN; P67870; B0UXA9; P07312; P13862; Q4VX47 

Genbank Protein ID

 X16937; X16312; M30448; X57152; AY113186; CR541699; AF129756; BA000025; DQ314868; AK311860; AL662899; AL662899; AL670886; AL805934; BX511262; CR753842; CR354443; CR759761; CH471081; BC112017; BC112019 

Genbank Nucleotide ID

 CAA34811.1; CAA34379.1; AAA52123.1; CAA40442.1; AAM50092.1; CAG46500.1; AAD18081.1; BAB63386.1; ABC40727.1; BAG34801.1; CAI96141.1; CAI18393.2; CAI17800.1; CAI18523.1; CAM45825.1; CAQ06572.1; CAQ07002.1; CAQ10879.1; EAX03473.1; AAI12018.1; AAI12020.1 

Protein Name

 Casein kinase II subunit beta 

Protein Synonyms/Alias

 CK II beta; Phosvitin; Protein G5a 

Gene Name

 CSNK2B 

Gene Synonyms/Alias

 CK2N; G5A 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
33	DEDYIQDKFNLTGLN	ubiquitination	[1]
100	GIAQMLEKYQQGDFG	ubiquitination	[1, 2, 3, 4]
134	IPGEAMVKLYCPKCM	ubiquitination	[1, 3]
139	MVKLYCPKCMDVYTP	ubiquitination	[3, 5]
147	CMDVYTPKSSRHHHT	acetylation	[4]
147	CMDVYTPKSSRHHHT	ubiquitination	[2, 3, 4]
191	VPRLYGFKIHPMAYQ	ubiquitination	[1, 3, 6]
208	LQAASNFKSPVKTIR	ubiquitination	[1, 3, 5]
212	SNFKSPVKTIR****	acetylation	[4, 7]
212	SNFKSPVKTIR****	ubiquitination	[3, 4, 5]

Reference

 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit. 

Sequence Annotation

 REGION 188 193 Interaction with alpha subunit (By
 METAL 109 109 Zinc.
 METAL 114 114 Zinc.
 METAL 137 137 Zinc.
 METAL 140 140 Zinc.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine; by autocatalysis.
 MOD_RES 3 3 Phosphoserine; by autocatalysis.
 MOD_RES 209 209 Phosphoserine.
 MOD_RES 212 212 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Metal-binding; Phosphoprotein; Reference proteome; Wnt signaling pathway; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 215 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005956; C:protein kinase CK2 complex; NAS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0019887; F:protein kinase regulator activity; NAS:UniProtKB.
 GO:0033211; P:adiponectin-mediated signaling pathway; IDA:BHF-UCL.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0043623; P:cellular protein complex assembly; NAS:BHF-UCL.
 GO:0061154; P:endothelial tube morphogenesis; IMP:BHF-UCL.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0043537; P:negative regulation of blood vessel endothelial cell migration; IDA:BHF-UCL.
 GO:0008285; P:negative regulation of cell proliferation; TAS:BHF-UCL.
 GO:0032927; P:positive regulation of activin receptor signaling pathway; IMP:BHF-UCL.
 GO:0010862; P:positive regulation of pathway-restricted SMAD protein phosphorylation; IDA:BHF-UCL.
 GO:0051101; P:regulation of DNA binding; NAS:BHF-UCL.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 

Interpro

 IPR016149; Casein_kin_II_reg-sub_a-hlx.
 IPR016150; Casein_kin_II_reg-sub_b-sht.
 IPR000704; Casein_kinase_II_reg-sub. 

Pfam

 PF01214; CK_II_beta 

SMART

 SM01085; CK_II_beta 

PROSITE

 PS01101; CK2_BETA 

PRINTS

 PR00472; CASNKINASEII.