CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023630
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nck-associated protein 1 
Protein Synonyms/Alias
 NAP 1; Membrane-associated protein HEM-2; p125Nap1 
Gene Name
 NCKAP1 
Gene Synonyms/Alias
 HEM2; KIAA0587; NAP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12VLQPSQQKLAEKLTIubiquitination[1]
16SQQKLAEKLTILNDRubiquitination[1]
507QAYTSVSKASLGLADubiquitination[1]
557FYSRAFEKMFQQCLEubiquitination[1]
892QMRTSFDKPDQMAALubiquitination[1]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. 
Sequence Annotation
 MOD_RES 2 2 N-acetylserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Cell membrane; Cell projection; Complete proteome; Membrane; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1128 AA 
Protein Sequence
MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKQGQV WKACGDPKAK PSYLIDKNLE 60
SAVKFIVRKF PAVETRNNNQ QLAQLQKEKS EILKNLALYY FTFVDVMEFK DHVCELLNTI 120
DVCQVFFDIT VNFDLTKNYL DLIITYTTLM ILLSRIEERK AIIGLYNYAH EMTHGASDRE 180
YPRLGQMIVD YENPLKKMME EFVPHSKSLS DALISLQMVY PRRNLSADQW RNAQLLSLIS 240
APSTMLNPAQ SDTMPCEYLS LDAMEKWIIF GFILCHGILN TDATALNLWK LALQSSSCLS 300
LFRDEVFHIH KAAEDLFVNI RGYNKRINDI RECKEAAVSH AGSMHRERRK FLRSALKELA 360
TVLSDQPGLL GPKALFVFMA LSFARDEIIW LLRHADNMPK KSADDFIDKH IAELIFYMEE 420
LRAHVRKYGP VMQRYYVQYL SGFDAVVLNE LVQNLSVCPE DESIIMSSFV NTMTSLSVKQ 480
VEDGEVFDFR GMRLDWFRLQ AYTSVSKASL GLADHRELGK MMNTIIFHTK MVDSLVEMLV 540
ETSDLSIFCF YSRAFEKMFQ QCLELPSQSR YSIAFPLLCT HFMSCTHELC PEERHHIGDR 600
SLSLCNMFLD EMAKQARNLI TDICTEQCTL SDQLLPKHCA KTISQAVNKK SKKQTGKKGE 660
PEREKPGVES MRKNRLVVTN LDKLHTALSE LCFSINYVPN MVVWEHTFTP REYLTSHLEI 720
RFTKSIVGMT MYNQATQEIA KPSELLTSVR AYMTVLQSIE NYVQIDITRV FNNVLLQQTQ 780
HLDSHGEPTI TSLYTNWYLE TLLRQVSNGH IAYFPAMKAF VNLPTENELT FNAEEYSDIS 840
EMRSLSELLG PYGMKFLSES LMWHISSQVA ELKKLVVENV DVLTQMRTSF DKPDQMAALF 900
KRLSSVDSVL KRMTIIGVIL SFRSLAQEAL RDVLSYHIPF LVSSIEDFKD HIPRETDMKV 960
AMNVYELSSA AGLPCEIDPA LVVALSSQKS ENISPEEEYK IACLLMVFVA VSLPTLASNV 1020
MSQYSPAIEG HCNNIHCLAK AINQIAAALF TIHKGSIEDR LKEFLALASS SLLKIGQETD 1080
KTTTRNRESV YLLLDMIVQE SPFLTMDLLE SCFPYVLLRN AYHAVYKQSV TSSA 1134 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031258; C:lamellipodium membrane; IEA:UniProtKB-SubCell.
 GO:0045176; P:apical protein localization; IEA:Compara.
 GO:0006915; P:apoptotic process; TAS:ProtInc.
 GO:0045175; P:basal protein localization; IEA:Compara.
 GO:0007417; P:central nervous system development; TAS:ProtInc.
 GO:0010172; P:embryonic body morphogenesis; IEA:Compara.
 GO:0048617; P:embryonic foregut morphogenesis; IEA:Compara.
 GO:0035050; P:embryonic heart tube development; IEA:Compara.
 GO:0007492; P:endoderm development; IEA:Compara.
 GO:0030950; P:establishment or maintenance of actin cytoskeleton polarity; IEA:Compara.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0030032; P:lamellipodium assembly; IEA:Compara.
 GO:0008078; P:mesodermal cell migration; IEA:Compara.
 GO:0001843; P:neural tube closure; IEA:Compara.
 GO:0048570; P:notochord morphogenesis; IEA:Compara.
 GO:0048340; P:paraxial mesoderm morphogenesis; IEA:Compara.
 GO:0050821; P:protein stabilization; IEA:Compara.
 GO:0032880; P:regulation of protein localization; IEA:Compara.
 GO:0001756; P:somitogenesis; IEA:Compara.
 GO:0007354; P:zygotic determination of anterior/posterior axis, embryo; IEA:Compara. 
Interpro
 IPR019137; Nck-associated_protein-1. 
Pfam
 PF09735; Nckap1 
SMART
  
PROSITE
  
PRINTS