CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014224
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Paired amphipathic helix protein Sin3a 
Protein Synonyms/Alias
 Histone deacetylase complex subunit Sin3a; Transcriptional corepressor Sin3a 
Gene Name
 Sin3a 
Gene Synonyms/Alias
 Kiaa4126 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
639RVLEAIQKKLSRLSAacetylation[1]
866GGSPPKSKLLFSNTAacetylation[2]
876FSNTAAQKLRGMDEVacetylation[2, 3]
876FSNTAAQKLRGMDEVubiquitination[4]
938VLGIKRDKSDSPAIQubiquitination[4]
Reference
 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. 
Sequence Annotation
 DOMAIN 119 189 PAH 1.
 DOMAIN 300 383 PAH 2.
 DOMAIN 457 526 PAH 3.
 REGION 119 196 Interaction with HCFC1 (By similarity).
 REGION 205 479 Interaction with REST.
 REGION 459 526 Interaction with SAP30.
 REGION 524 851 Interaction with NCOR1.
 REGION 525 660 Interactions with SUDS3 and SAP130 (By
 REGION 688 830 Interactions with HDAC1 and ARID4B (By
 REGION 889 968 Interaction with OGT (By similarity).
 MOD_RES 10 10 Phosphoserine (By similarity).
 MOD_RES 277 277 Phosphoserine (By similarity).
 MOD_RES 470 470 N6-acetyllysine (By similarity).
 MOD_RES 833 833 Phosphoserine (By similarity).
 MOD_RES 861 861 Phosphoserine.
 MOD_RES 941 941 Phosphoserine (By similarity).
 MOD_RES 1113 1113 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Alternative splicing; Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1274 AA 
Protein Sequence
MKRRLDDQES PVYAAQQRRI PGSTEAFSHQ HRVLAPAPPV YEAVSETMQS ATGIQYSVAP 60
NYQVSAVPQS SGSHGPAIAA VHSSHHHPTA VQPHGGQVVQ SHAHPAPPVA PVQGQQQFQR 120
LKVEDALSYL DQVKLQFGSQ PQVYNDFLDI MKEFKSQSID TPGVISRVSQ LFKGHPDLIM 180
GFNTFLPPGY KIEVQTNDMV NVTTPGQVHQ IPTHGIQPQP QPPPQHPSQP SSQSAPTPAQ 240
PAPQPTAAKV SKPSQLQAHT PASQQTPPLP PYASPRSPPV QPHTPVTISL GTAPSLQNNQ 300
PVEFNHAINY VNKIKNRFQG QPDIYKAFLE ILHTYQKEQR NAKEAGGNYT PALTEQEVYA 360
QVARLFKNQE DLLSEFGQFL PDANSSVLLS KTTAEKVDSV RNDHGGTVKK PQLNNKPQRP 420
SQNGCQIRRH SGTGATPPVK KKPKLMSLKE SSMADASKHG VGTESLFFDK VRKALRSAEA 480
YENFLRCLVI FNQEVISRAE LVQLVSPFLG KFPELFNWFK NFLGYKESVH LESFPKERAT 540
EGIAMEIDYA SCKRLGSSYR ALPKSYQQPK CTGRTPLCKE VLNDTWVSFP SWSEDSTFVS 600
SKKTQYEEHI YRCEDERFEL DVVLETNLAT IRVLEAIQKK LSRLSAEEQA KFRLDNTLGG 660
TSEVIHRKAL QRIYADKAAD IIDGLRKNPS IAVPIVLKRL KMKEEEWREA QRGFNKVWRE 720
QNEKYYLKSL DHQGINFKQN DTKVLRSKSL LNEIESIYDE RQEQATEENA GVPVGPHLSL 780
AYEDKQILED AAALIIHHVK RQTGIQKEDK YKIKQIMHHF IPDLLFAQRG DLSDVEEEEE 840
EEMDVDEATG APKKHNGVGG SPPKSKLLFS NTAAQKLRGM DEVYNLFYVN NNWYIFMRLH 900
QILCLRLLRI CSQAERQIEE ENREREWERE VLGIKRDKSD SPAIQLRLKE PMDVDVEDYY 960
PAFLDMVRSL LDGNIDSSQY EDSLREMFTI HAYIAFTMDK LIQSIVRQLQ HIVSDEVCVQ 1020
VTDLYLAENN NGATGGQLNS QTSRSLLESA YQRKAEQLMS DENCFKLMFI QSQGQVQLTV 1080
ELLDTEEENS DDPVEAERWS DYVERYMSSD TTSPELREHL AQKPVFLPRN LRRIRKCQRG 1140
REQQEKEGKE GNSKKTMENV ESLDKLECRF KLNSYKMVYV IKSEDYMYRR TALLRAHQSH 1200
ERVSKRLHQR FQAWVDKWTK EHVPREMAAE TSKWLMGEGL EGLVPCTTTC DTETLHFVSI 1260
NKYRVKYGTV FKAP 1274 
Gene Ontology
 GO:0000776; C:kinetochore; IDA:MGI.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0016580; C:Sin3 complex; IEA:Compara.
 GO:0005667; C:transcription factor complex; IPI:MGI.
 GO:0017053; C:transcriptional repressor complex; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0003677; F:DNA binding; IDA:MGI.
 GO:0033558; F:protein deacetylase activity; IEA:Compara.
 GO:0001106; F:RNA polymerase II transcription corepressor activity; IGI:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IMP:MGI.
 GO:0002218; P:activation of innate immune response; IEA:Compara.
 GO:0007568; P:aging; IEA:Compara.
 GO:0034613; P:cellular protein localization; IMP:MGI.
 GO:0071333; P:cellular response to glucose stimulus; IDA:MGI.
 GO:0006260; P:DNA replication; IMP:MGI.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
 GO:1901675; P:negative regulation of histone H3-K27 acetylation; IEA:Compara.
 GO:1900181; P:negative regulation of protein localization to nucleus; IEA:Compara.
 GO:2000678; P:negative regulation of transcription regulatory region DNA binding; IEA:Compara.
 GO:0031937; P:positive regulation of chromatin silencing; IEA:Compara.
 GO:0002230; P:positive regulation of defense response to virus by host; IEA:Compara.
 GO:0010971; P:positive regulation of G2/M transition of mitotic cell cycle; IMP:MGI.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0043619; P:regulation of transcription from RNA polymerase II promoter in response to oxidative stress; IEA:Compara.
 GO:0051595; P:response to methylglyoxal; IDA:MGI.
 GO:0010243; P:response to organic nitrogen; IEA:Compara. 
Interpro
 IPR013194; HDAC_interact.
 IPR003822; PAH. 
Pfam
 PF02671; PAH
 PF08295; Sin3_corepress 
SMART
 SM00761; HDAC_interact 
PROSITE
 PS51477; PAH 
PRINTS