CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012490
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein OZF 
Protein Synonyms/Alias
 Only zinc finger protein; Zinc finger protein 146 
Gene Name
 ZNF146 
Gene Synonyms/Alias
 OZF 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19GENPFACKVCGKVFSubiquitination[1]
42EHFHTREKPFECNECubiquitination[2]
56CGKAFSQKQYVIKHQubiquitination[2, 3]
70QNTHTGEKLFECNECubiquitination[2]
79FECNECGKSFSQKENubiquitination[2]
84CGKSFSQKENLLTHQubiquitination[1, 2]
103GEKPFECKDCGKAFIubiquitination[2]
112CGKAFIQKSNLIRHQubiquitination[1, 2, 3, 4, 5, 6]
126QRTHTGEKPFVCKECubiquitination[2]
131GEKPFVCKECGKTFSubiquitination[2]
140CGKTFSGKSNLTEHEubiquitination[2]
169GTAFGQKKYLIKHQNubiquitination[2]
173GQKKYLIKHQNIHTGubiquitination[2]
182QNIHTGEKPYECNECubiquitination[2]
191YECNECGKAFSQRTSubiquitination[2]
210VRIHSGDKPYECNVCubiquitination[2]
238VRSHTGEKPYGCNECubiquitination[2]
265HLRIHTGKKPYQCSEubiquitination[2]
266LRIHTGKKPYQCSECubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 ZN_FING 16 38 C2H2-type 1.
 ZN_FING 44 66 C2H2-type 2.
 ZN_FING 72 94 C2H2-type 3.
 ZN_FING 100 122 C2H2-type 4.
 ZN_FING 128 150 C2H2-type 5.
 ZN_FING 156 178 C2H2-type 6.
 ZN_FING 184 206 C2H2-type 7.
 ZN_FING 212 234 C2H2-type 8.
 ZN_FING 240 262 C2H2-type 9.
 ZN_FING 268 290 C2H2-type 10.
 REGION 212 292 Interaction with TERF2IP.
 CROSSLNK 157 157 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 169 169 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Polymorphism; Reference proteome; Repeat; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 292 AA 
Protein Sequence
MSHLSQQRIY SGENPFACKV CGKVFSHKSN LTEHEHFHTR EKPFECNECG KAFSQKQYVI 60
KHQNTHTGEK LFECNECGKS FSQKENLLTH QKIHTGEKPF ECKDCGKAFI QKSNLIRHQR 120
THTGEKPFVC KECGKTFSGK SNLTEHEKIH IGEKPFKCSE CGTAFGQKKY LIKHQNIHTG 180
EKPYECNECG KAFSQRTSLI VHVRIHSGDK PYECNVCGKA FSQSSSLTVH VRSHTGEKPY 240
GCNECGKAFS QFSTLALHLR IHTGKKPYQC SECGKAFSQK SHHIRHQKIH TH 292 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0008201; F:heparin binding; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; TAS:ProtInc.
 GO:0006355; P:regulation of transcription, DNA-dependent; TAS:ProtInc. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS