CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007200
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Osmosensing histidine protein kinase SLN1 
Protein Synonyms/Alias
 Osmolarity two-component system protein SLN1; Tyrosine phosphatase-dependent protein 2 
Gene Name
 SLN1 
Gene Synonyms/Alias
 YPD2; YIL147C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
794VANESIYKKVNDREKubiquitination[1]
913GTMKLESKVGVGSKFubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Histidine kinase that acts as a osmosensor at the plasma membrane. Part of the bifurcated SLN1-YPD1-SKN7/SSK1 two-component regulatory system, which controls activity of the HOG1 pathway and gene expression in response to changes in the osmolarity of the extracellular environment. Under normal osmotic conditions, the histidine kinase autophosphorylates His-576. This phosphate is subsequently transferred to Asp-1144, from where it is relayed to 'His-64' of the phosphorelay intermediate protein YPD1. Under high osmolarity conditions, the histidine kinase is no longer active. 
Sequence Annotation
 DOMAIN 573 928 Histidine kinase.
 DOMAIN 1089 1210 Response regulatory.
 METAL 1094 1094 Magnesium (By similarity).
 METAL 1095 1095 Magnesium (By similarity).
 METAL 1144 1144 Magnesium (By similarity).
 METAL 1195 1195 Magnesium (By similarity).
 MOD_RES 502 502 Phosphoserine.
 MOD_RES 576 576 Phosphohistidine; by autocatalysis.
 MOD_RES 758 758 Phosphoserine.
 MOD_RES 833 833 Phosphoserine.
 MOD_RES 1041 1041 Phosphoserine.
 MOD_RES 1044 1044 Phosphoserine.
 MOD_RES 1144 1144 4-aspartylphosphate.
 CARBOHYD 100 100 N-linked (GlcNAc...) (Potential).
 CARBOHYD 138 138 N-linked (GlcNAc...) (Potential).
 CARBOHYD 142 142 N-linked (GlcNAc...) (Potential).
 CARBOHYD 181 181 N-linked (GlcNAc...) (Potential).
 CARBOHYD 224 224 N-linked (GlcNAc...) (Potential).
 CARBOHYD 272 272 N-linked (GlcNAc...) (Potential).  
Keyword
 3D-structure; ATP-binding; Cell membrane; Complete proteome; Glycoprotein; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Transmembrane; Transmembrane helix; Two-component regulatory system. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1220 AA 
Protein Sequence
MRFGLPSKLE LTPPFRIGIR TQLTALVSIV ALGSLIILAV TTGVYFTSNY KNLRSDRLYI 60
AAQLKSSQID QTLNYLYYQA YYLASRDALQ SSLTSYVAGN KSADNWVDSL SVIQKFLSSS 120
NLFYVAKVYD SSFNAVLNAT NNGTGDLIPE DVLDSLFPLS TDTPLPSSLE TIGILTDPVL 180
NSTDYLMSMS LPIFANPSII LTDSRVYGYI TIIMSAEGLK SVFNDTTALE HSTIAIISAV 240
YNSQGKASGY HFVFPPYGSR SDLPQKVFSI KNDTFISSAF RNGKGGSLKQ TNILSTRNTA 300
LGYSPCSFNL VNWVAIVSQP ESVFLSPATK LAKIITGTVI AIGVFVILLT LPLAHWAVQP 360
IVRLQKATEL ITEGRGLRPS TPRTISRASS FKRGFSSGFA VPSSLLQFNT AEAGSTTSVS 420
GHGGSGHGSG AAFSANSSMK SAINLGNEKM SPPEEENKIP NNHTDAKISM DGSLNHDLLG 480
PHSLRHNDTD RSSNRSHILT TSANLTEARL PDYRRLFSDE LSDLTETFNT MTDALDQHYA 540
LLEERVRART KQLEAAKIEA EAANEAKTVF IANISHELRT PLNGILGMTA ISMEETDVNK 600
IRNSLKLIFR SGELLLHILT ELLTFSKNVL QRTKLEKRDF CITDVALQIK SIFGKVAKDQ 660
RVRLSISLFP NLIRTMVLWG DSNRIIQIVM NLVSNALKFT PVDGTVDVRM KLLGEYDKEL 720
SEKKQYKEVY IKKGTEVTEN LETTDKYDLP TLSNHRKSVD LESSATSLGS NRDTSTIQEE 780
ITKRNTVANE SIYKKVNDRE KASNDDVSSI VSTTTSSYDN AIFNSQFNKA PGSDDEEGGN 840
LGRPIENPKT WVISIEVEDT GPGIDPSLQE SVFHPFVQGD QTLSRQYGGT GLGLSICRQL 900
ANMMHGTMKL ESKVGVGSKF TFTLPLNQTK EISFADMEFP FEDEFNPESR KNRRVKFSVA 960
KSIKSRQSTS SVATPATNRS SLTNDVLPEV RSKGKHETKD VGNPNMGREE KNDNGGLEQL 1020
QEKNIKPSIC LTGAEVNEQN SLSSKHRSRH EGLGSVNLDR PFLQSTGTAT SSRNIPTVKD 1080
DDKNETSVKI LVVEDNHVNQ EVIKRMLNLE GIENIELACD GQEAFDKVKE LTSKGENYNM 1140
IFMDVQMPKV DGLLSTKMIR RDLGYTSPIV ALTAFADDSN IKECLESGMN GFLSKPIKRP 1200
KLKTILTEFC AAYQGKKNNK 1220 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0009927; F:histidine phosphotransfer kinase activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005034; F:osmosensor activity; IDA:SGD.
 GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
 GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
 GO:0004673; F:protein histidine kinase activity; IDA:SGD.
 GO:0035556; P:intracellular signal transduction; IEA:GOC.
 GO:0006469; P:negative regulation of protein kinase activity; IMP:SGD.
 GO:0007234; P:osmosensory signaling via phosphorelay pathway; IDA:SGD.
 GO:0018106; P:peptidyl-histidine phosphorylation; IDA:SGD.
 GO:0046777; P:protein autophosphorylation; IDA:SGD.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro.
 GO:0023014; P:signal transduction by phosphorylation; IEA:GOC. 
Interpro
 IPR011006; CheY-like_superfamily.
 IPR003594; HATPase_ATP-bd.
 IPR004358; Sig_transdc_His_kin-like_C.
 IPR003661; Sig_transdc_His_kin_sub1_dim/P.
 IPR005467; Sig_transdc_His_kinase_core.
 IPR009082; Sig_transdc_His_kinase_dimeric.
 IPR001789; Sig_transdc_resp-reg_receiver. 
Pfam
 PF02518; HATPase_c
 PF00512; HisKA
 PF00072; Response_reg 
SMART
 SM00387; HATPase_c
 SM00388; HisKA
 SM00448; REC 
PROSITE
 PS50109; HIS_KIN
 PS50110; RESPONSE_REGULATORY 
PRINTS
 PR00344; BCTRLSENSOR.