CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-045281
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Rho guanine nucleotide exchange factor 1 
Protein Synonyms/Alias
  
Gene Name
 ARHGEF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
170DTLHSLPKSQVKRQEubiquitination[1]
268AEGSWFQKISSRFCSubiquitination[1, 2, 3, 4, 5, 6]
313RCRRLQLKDMIPTEMubiquitination[1]
325TEMQRLTKYPLLLQSubiquitination[1, 3, 4, 5, 6]
345EEPTEREKVELAAECubiquitination[1, 6]
397DPMLSEFKNLDITKKubiquitination[1, 3]
403FKNLDITKKKLVHEGubiquitination[1]
404KNLDITKKKLVHEGPubiquitination[1]
405NLDITKKKLVHEGPLubiquitination[1, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 702 AA 
Protein Sequence
XGVGMPSRDR NIGAPGQDTP GVSLHPLSLD SPDREPGADA PLELGDSSPQ GPMSLESLAP 60
PESTDEGAET ERRWKRLSGR LGRSESLRVS DRRRPSRGSL GAKGRGGGRS RSDVDMDPSS 120
ATAVLGPARR ATPEPGDEGE PGRSGLELEP EEPPGWRELV PPDTLHSLPK SQVKRQEVIS 180
ELLVTEAAHV RMLRVLHDLF FQPMAECLFF PLEELQNIFP SLDELIEVHS LFLDRLMKRR 240
QESGYLIEEI GDVLLARFDG AEGSWFQKIS SRFCSRQSFA LEQLKAKQRK DPRFCAFVQE 300
AESRPRCRRL QLKDMIPTEM QRLTKYPLLL QSIGQNTEEP TEREKVELAA ECCREILHHV 360
NQAVRDMEDL LRLKDYQRRL DLSHLRQSSD PMLSEFKNLD ITKKKLVHEG PLTWRVTKDK 420
AVEVHVLLLD DLLLLLQRQD ERLLLKSHSR TLTPTPDGKT MLRPVLRLTS AMTREVATDH 480
KAFYVLFTWD QEAQIYELVA QTVSERKNWC ALITETAGSL KVPAPASRPK PRPSPSSTRE 540
PLLSSSENGN GGRETSPADA RTERILSDLL PFCRPGPEGQ LAATALRKGV GGGILPPETP 600
PVSAWGELCP PAWLHLRFPP RKHPILTTTC EVGTAMMDAH FTDVETEVLT GKVTWPKSHR 660
DEVSLDLNAG ARWRWRRCVQ DPCWTRKPGP GLEHGPAHTQ PG 702 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro. 
Interpro
 IPR000219; DH-domain.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR015721; RhoGEF-like. 
Pfam
 PF00621; RhoGEF 
SMART
 SM00233; PH
 SM00325; RhoGEF 
PROSITE
 PS50010; DH_2
 PS50003; PH_DOMAIN 
PRINTS