UniProt Accession

 SRSF2_HUMAN; Q01130; B3KWD5 

Genbank Protein ID

 M90104; X62447; X75755; BT007250; AK124792; CH471099; BC000339; BC001303; BC070086; L03693 

Genbank Nucleotide ID

 AAA60306.1; CAA44307.1; CAA53383.1; AAP35914.1; BAG54097.1; EAW89439.1; AAH00339.1; AAH01303.1; AAH70086.1; AAA60162.1 

Protein Name

 Serine/arginine-rich splicing factor 2 

Protein Synonyms/Alias

 Protein PR264; Splicing component, 35 kDa; Splicing factor SC35; SC-35; Splicing factor, arginine/serine-rich 2 

Gene Name

 SRSF2 

Gene Synonyms/Alias

 SFRS2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
36	TLRRVFEKYGRVGDV	acetylation	[1]
36	TLRRVFEKYGRVGDV	ubiquitination	[2, 3]
52	IPRDRYTKESRGFAF	acetylation	[4]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Acetylation and phosphorylation of SRSF2 control cell fate decision in response to cisplatin.
 Edmond V, Moysan E, Khochbin S, Matthias P, Brambilla C, Brambilla E, Gazzeri S, Eymin B.
 EMBO J. 2011 Feb 2;30(3):510-23. [PMID: 21157427] 

Functional Description

 Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment. 

Sequence Annotation

 DOMAIN 14 92 RRM.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 22 22 Phosphothreonine.
 MOD_RES 25 25 Phosphothreonine.
 MOD_RES 26 26 Phosphoserine.
 MOD_RES 52 52 N6-acetyllysine.
 MOD_RES 187 187 Phosphoserine (By similarity).
 MOD_RES 189 189 Phosphoserine.
 MOD_RES 191 191 Phosphoserine.
 MOD_RES 204 204 Phosphoserine.
 MOD_RES 206 206 Phosphoserine.
 MOD_RES 208 208 Phosphoserine.
 MOD_RES 212 212 Phosphoserine.
 MOD_RES 220 220 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 221 AA 

Protein Sequence

Gene Ontology

 GO:0016607; C:nuclear speck; IDA:UniProtKB.
 GO:0005681; C:spliceosomal complex; IEA:Compara.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003714; F:transcription corepressor activity; NAS:UniProtKB.
 GO:0031124; P:mRNA 3'-end processing; TAS:Reactome.
 GO:0006406; P:mRNA export from nucleus; TAS:Reactome.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS