CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004604
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glucokinase-1 
Protein Synonyms/Alias
 Glucose kinase 1; GLK-1 
Gene Name
 GLK1 
Gene Synonyms/Alias
 HOR3; YCL040W; YCL312; YCL40W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
150YHPDELAKGKDAKPMacetylation[1]
267EEINKITKLPQELRDacetylation[1]
304LKHLPTTKYDVVIDQacetylation[1]
324PGFHLFEKRVSGMFLacetylation[1]
355LLQQYRSKEQLPRHLubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Two isoenzymes, hexokinase-1 and hexokinase-2, can phosphorylate keto- and aldohexoses in yeast, whereas a third isoenzyme, GLK, is specific for aldohexoses. All glucose phosphorylating enzymes are involved in glucose uptake. 
Sequence Annotation
 NP_BIND 487 492 ATP (Potential).
 REGION 158 184 Glucose-binding (Potential).
 BINDING 110 110 ATP (Potential).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 470 470 Phosphoserine.  
Keyword
 Acetylation; ATP-binding; Complete proteome; Glycolysis; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 500 AA 
Protein Sequence
MSFDDLHKAT ERAVIQAVDQ ICDDFEVTPE KLDELTAYFI EQMEKGLAPP KEGHTLASDK 60
GLPMIPAFVT GSPNGTERGV LLAADLGGTN FRICSVNLHG DHTFSMEQMK SKIPDDLLDD 120
ENVTSDDLFG FLARRTLAFM KKYHPDELAK GKDAKPMKLG FTFSYPVDQT SLNSGTLIRW 180
TKGFRIADTV GKDVVQLYQE QLSAQGMPMI KVVALTNDTV GTYLSHCYTS DNTDSMTSGE 240
ISEPVIGCIF GTGTNGCYME EINKITKLPQ ELRDKLIKEG KTHMIINVEW GSFDNELKHL 300
PTTKYDVVID QKLSTNPGFH LFEKRVSGMF LGEVLRNILV DLHSQGLLLQ QYRSKEQLPR 360
HLTTPFQLSS EVLSHIEIDD STGLRETELS LLQSLRLPTT PTERVQIQKL VRAISRRSAY 420
LAAVPLAAIL IKTNALNKRY HGEVEIGCDG SVVEYYPGFR SMLRHALALS PLGAEGERKV 480
HLKIAKDGSG VGAALCALVA 500 
Gene Ontology
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004340; F:glucokinase activity; IDA:SGD.
 GO:0046323; P:glucose import; IGI:SGD.
 GO:0006096; P:glycolysis; IDA:SGD.
 GO:0006013; P:mannose metabolic process; IDA:SGD. 
Interpro
 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 
Pfam
 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 
SMART
  
PROSITE
 PS00378; HEXOKINASES 
PRINTS
 PR00475; HEXOKINASE.