CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016432
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calcium-binding mitochondrial carrier protein Aralar1 
Protein Synonyms/Alias
 Mitochondrial aspartate glutamate carrier 1; Solute carrier family 25 member 12 
Gene Name
 Slc25a12 
Gene Synonyms/Alias
 Aralar1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
55NDPNSNPKIVQLLAGubiquitination[1]
377KNSFDCFKKVLRYEGacetylation[2, 3, 4]
378NSFDCFKKVLRYEGFacetylation[2]
403LIGVAPEKAIKLTVNacetylation[2, 3, 4, 5]
406VAPEKAIKLTVNDFVacetylation[3]
406VAPEKAIKLTVNDFVubiquitination[1]
485FGLYKGAKACFLRDIacetylation[2]
543VTPADVIKTRLQVAAacetylation[3]
578EGPSAFWKGTAARVFacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199] 
Functional Description
 Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle (By similarity). 
Sequence Annotation
 DOMAIN 40 85 EF-hand 1.
 DOMAIN 86 121 EF-hand 2.
 DOMAIN 122 156 EF-hand 3.
 DOMAIN 157 192 EF-hand 4.
 REPEAT 324 416 Solcar 1.
 REPEAT 424 508 Solcar 2.
 REPEAT 516 604 Solcar 3.  
Keyword
 Calcium; Complete proteome; Direct protein sequencing; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 677 AA 
Protein Sequence
MAVKVHTTKR GDPHELRNIF LQYASTEVDG EHYMTPEDFV QRYLGLYNDP NSNPKIVQLL 60
AGVADQTKDG LISYQEFLAF ESVLCAPDSM FIVAFQLFDK SGNGEVTFEN VKEIFGQTII 120
HHHIPFNWDC EFIRLHFGHN RKKHLNYVEF TQFLQELQLE HARQAFALKD KSKSGMISGL 180
DFSDVMVTIR SHMLTPFVEE NLVSAAGGGT SHQVSFSYFN AFNSLLNNME LVRKIYSTLA 240
GTRKDIEVTK EEFAQSAIRY GQVTPLEIDI LYQLADLYNA SGRLTLADIE RIAPLAEGAL 300
PYNLAELQRQ QSPGLGRPIW LQIAESAYRF TLGSVAGAVG ATAVYPIDLV KTRMQNQRGT 360
GSVVGELMYK NSFDCFKKVL RYEGFFGLYR GLIPQLIGVA PEKAIKLTVN DFVRDKFTKR 420
DGSIPLPAEI LAGGCAGGSQ VIFTNPLEIV KIRLQVAGEI TTGPRVSALN VLQDLGLFGL 480
YKGAKACFLR DIPFSAIYFP VYAHCKLLLA DENGRVGGIN LLTAGALAGV PAASLVTPAD 540
VIKTRLQVAA RAGQTTYSGV VDCFRKILRE EGPSAFWKGT AARVFRSSPQ FGVTLVTYEL 600
LQRWFYIDFG GLKPSGSEPT PKSRIADLPP ANPDHIGGYR LATATFAGIE NKFGLYLPKF 660
KSPSVAVAQP KAAAAAQ 677 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005509; F:calcium ion binding; ISS:UniProtKB.
 GO:0015183; F:L-aspartate transmembrane transporter activity; ISS:UniProtKB.
 GO:0005313; F:L-glutamate transmembrane transporter activity; ISS:UniProtKB.
 GO:0043490; P:malate-aspartate shuttle; ISS:UniProtKB.
 GO:0051592; P:response to calcium ion; ISS:UniProtKB. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR002067; Mit_carrier.
 IPR018108; Mitochondrial_sb/sol_carrier.
 IPR023395; Mt_carrier_dom. 
Pfam
 PF13405; EF_hand_4
 PF00153; Mito_carr 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50920; SOLCAR 
PRINTS
 PR00926; MITOCARRIER.