CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004603
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 S-adenosylmethionine decarboxylase proenzyme 
Protein Synonyms/Alias
 AdoMetDC; SAMDC; S-adenosylmethionine decarboxylase alpha chain; S-adenosylmethionine decarboxylase beta chain 
Gene Name
 AMD1 
Gene Synonyms/Alias
 AMD 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
96KALVPLLKLARDYSGubiquitination[1, 2, 3]
201MKDGVTAKDVTRESGubiquitination[2, 4]
291LFVNQSSKCRTVLASubiquitination[4]
301TVLASPQKIEGFKRLubiquitination[1, 3, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
 ACT_SITE 8 8
 ACT_SITE 11 11
 ACT_SITE 68 68 Schiff-base intermediate with substrate;
 ACT_SITE 82 82 Proton donor; for catalytic activity.
 ACT_SITE 229 229 Proton acceptor; for processing activity.
 ACT_SITE 243 243 Proton acceptor; for processing activity.
 BINDING 7 7 Substrate.
 BINDING 67 67 Substrate.
 BINDING 223 223 Substrate.
 BINDING 247 247 Substrate.
 MOD_RES 68 68 Pyruvic acid (Ser); by autocatalysis.
 MOD_RES 298 298 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Autocatalytic cleavage; Complete proteome; Decarboxylase; Direct protein sequencing; Lyase; Phosphoprotein; Polyamine biosynthesis; Pyruvate; Reference proteome; S-adenosyl-L-methionine; Schiff base; Spermidine biosynthesis; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 334 AA 
Protein Sequence
MEAAHFFEGT EKLLEVWFSR QQPDANQGSG DLRTIPRSEW DILLKDVQCS IISVTKTDKQ 60
EAYVLSESSM FVSKRRFILK TCGTTLLLKA LVPLLKLARD YSGFDSIQSF FYSRKNFMKP 120
SHQGYPHRNF QEEIEFLNAI FPNGAAYCMG RMNSDCWYLY TLDFPESRVI SQPDQTLEIL 180
MSELDPAVMD QFYMKDGVTA KDVTRESGIR DLIPGSVIDA TMFNPCGYSM NGMKSDGTYW 240
TIHITPEPEF SYVSFETNLS QTSYDDLIRK VVEVFKPGKF VTTLFVNQSS KCRTVLASPQ 300
KIEGFKRLDC QSAMFNDYNF VFTSFAKKQQ QQQS 334 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0004014; F:adenosylmethionine decarboxylase activity; TAS:UniProtKB.
 GO:0006557; P:S-adenosylmethioninamine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0008295; P:spermidine biosynthetic process; NAS:UniProtKB.
 GO:0006597; P:spermine biosynthetic process; NAS:UniProtKB. 
Interpro
 IPR001985; S-AdoMet_decarboxylase.
 IPR018167; S-AdoMet_decarboxylase_subgr.
 IPR016067; S-AdoMet_deCO2ase_core.
 IPR018166; S-AdoMet_deCO2ase_CS. 
Pfam
 PF01536; SAM_decarbox 
SMART
  
PROSITE
 PS01336; ADOMETDC 
PRINTS