CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018257
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 General transcription factor 3C polypeptide 2 
Protein Synonyms/Alias
 TF3C-beta; Transcription factor IIIC 110 kDa subunit; TFIIIC 110 kDa subunit; TFIIIC110; Transcription factor IIIC subunit beta 
Gene Name
 GTF3C2 
Gene Synonyms/Alias
 KIAA0011 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
155KAELLLLKLSKDLDRubiquitination[1]
315WKCLHLTKDFREQKHubiquitination[1, 2]
354PYLPQEEKSPLFSVQubiquitination[1, 3]
474NGCIWDLKFCPSGAWubiquitination[4]
489ELPGTPRKAPLLPRLubiquitination[5]
602RLSDGSLKLYPFQCFubiquitination[1]
756ERRFPIYKADLIPYQubiquitination[1]
783SGVPNPPKARTYTETubiquitination[1]
839LQLEAIHKVRFSPNLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1. 
Sequence Annotation
 REPEAT 461 517 WD 1.
 REPEAT 548 589 WD 2.
 REPEAT 607 647 WD 3.
 REPEAT 828 870 WD 4.
 MOD_RES 63 63 Phosphoserine.
 MOD_RES 132 132 Phosphoserine.
 MOD_RES 165 165 Phosphoserine.
 MOD_RES 167 167 Phosphoserine.
 MOD_RES 220 220 Phosphoserine.
 MOD_RES 871 871 Phosphoserine.
 MOD_RES 892 892 Phosphoserine.
 MOD_RES 893 893 Phosphoserine.
 MOD_RES 895 895 Phosphothreonine.  
Keyword
 Alternative splicing; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 911 AA 
Protein Sequence
MDTCGVGYVA LGEAGPVGNM TVVDSPGQEV LNQLDVKTSS EMTSAEASVE MSLPTPLPGF 60
EDSPDQRRLP PEQESLSRLE QPDLSSEMSK VSKPRASKPG RKRGGRTRKG PKRPQQPNPP 120
SAPLVPGLLD QSNPLSTPMP KKRGRKSKAE LLLLKLSKDL DRPESQSPKR PPEDFETPSG 180
ERPRRRAAQV ALLYLQELAE ELSTALPAPV SCPEGPKVSS PTKPKKIRQP AACPGGEEVD 240
GAPRDEDFFL QVEAEDVEES EGPSESSSEP EPVVPRSTPR GSTSGKQKPH CRGMAPNGLP 300
NHIMAPVWKC LHLTKDFREQ KHSYWEFAEW IPLAWKWHLL SELEAAPYLP QEEKSPLFSV 360
QREGLPEDGT LYRINRFSSI TAHPERWDVS FFTGGPLWAL DWCPVPEGAG ASQYVALFSS 420
PDMNETHPLS QLHSGPGLLQ LWGLGTLQQE SCPGNRAHFV YGIACDNGCI WDLKFCPSGA 480
WELPGTPRKA PLLPRLGLLA LACSDGKVLL FSLPHPEALL AQQPPDAVKP AIYKVQCVAT 540
LQVGSMQATD PSECGQCLSL AWMPTRPHQH LAAGYYNGMV VFWNLPTNSP LQRIRLSDGS 600
LKLYPFQCFL AHDQAVRTLQ WCKANSHFLV SAGSDRKIKF WDLRRPYEPI NSIKRFLSTE 660
LAWLLPYNGV TVAQDNCYAS YGLCGIHYID AGYLGFKAYF TAPRKGTVWS LSGSDWLGTI 720
AAGDISGELI AAILPDMALN PINVKRPVER RFPIYKADLI PYQDSPEGPD HSSASSGVPN 780
PPKARTYTET VNHHYLLFQD TDLGSFHDLL RREPMLRMQE GEGHSQLCLD RLQLEAIHKV 840
RFSPNLDSYG WLVSGGQSGL VRIHFVRGLA SPLGHRMQLE SRAHFNAMFQ PSSPTRRPGF 900
SPTSHRLLPT P 911 
Gene Ontology
 GO:0000127; C:transcription factor TFIIIC complex; IDA:HGNC.
 GO:0042791; P:5S class rRNA transcription from RNA polymerase III type 1 promoter; IC:HGNC.
 GO:0042797; P:tRNA transcription from RNA polymerase III promoter; IC:HGNC. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS