CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016441
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Enoyl-CoA hydratase, mitochondrial 
Protein Synonyms/Alias
 Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH 
Gene Name
 Echs1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
101FAAGADIKEMQNRTFacetylation[1, 2]
101FAAGADIKEMQNRTFubiquitination[3]
115FQDCYSSKFLSHWDHacetylation[2]
204RISAQDAKQAGLVSKacetylation[2, 4, 5]
204RISAQDAKQAGLVSKsuccinylation[4]
204RISAQDAKQAGLVSKubiquitination[3]
211KQAGLVSKIFPVEKLacetylation[2]
228EAIQCAEKIASNSKIacetylation[2]
260TEGNKLEKRLFYSTFacetylation[2]
282GMTAFVEKRKANFKDacetylation[2]
288EKRKANFKDH*****acetylation[2]
Reference
 [1] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate (By similarity). 
Sequence Annotation
 REGION 98 101 Substrate binding (By similarity).
 BINDING 141 141 Substrate; via amide nitrogen (By
 MOD_RES 101 101 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Fatty acid metabolism; Lipid metabolism; Lyase; Mitochondrion; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 290 AA 
Protein Sequence
MAALRALLPR ACSSLLSSVR CPELRRFASG ANFQYIITEK KGKNSSVGLI QLNRPKALNA 60
LCNGLIEELN QALETFEQDP AVGAIVLTGG DKAFAAGADI KEMQNRTFQD CYSSKFLSHW 120
DHITRVKKPV IAAVNGYALG GGCELAMMCD IIYAGEKAQF GQPEILLGTI PGAGGTQRLT 180
RAVGKSLAME MVLTGDRISA QDAKQAGLVS KIFPVEKLVE EAIQCAEKIA SNSKIVVAMA 240
KESVNAAFEM TLTEGNKLEK RLFYSTFATD DRREGMTAFV EKRKANFKDH 290 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004300; F:enoyl-CoA hydratase activity; IEA:EC.
 GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway. 
Interpro
 IPR001753; Crotonase_core_superfam.
 IPR018376; Enoyl-CoA_hyd/isom_CS. 
Pfam
 PF00378; ECH 
SMART
  
PROSITE
 PS00166; ENOYL_COA_HYDRATASE 
PRINTS