CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008165
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cysteine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Cysteinyl-tRNA synthetase; CysRS 
Gene Name
 CARS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
122YNSLTRNKEVFIPQDubiquitination[1]
131VFIPQDGKKVTWYCCubiquitination[1]
165DILRRVLKDYFKFDVubiquitination[1]
231NETTDPDKKQMLERIubiquitination[1]
232ETTDPDKKQMLERIQubiquitination[1]
251LATEPLEKAVQSRLTubiquitination[1]
300SIFSKLPKFWEGDFHubiquitination[1]
363AKFASSEKHSYGKLVubiquitination[1]
368SEKHSYGKLVPEAVGubiquitination[1]
378PEAVGDQKALQEGEGubiquitination[1]
407PNDFALWKASKPGEPubiquitination[1]
506TIKDALKKHSARQLRubiquitination[1]
586DKKTAIHKALCDNVDubiquitination[1]
689RKIAREQKVPEILQLubiquitination[1]
723EGLPTVVKLVDRNTLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
 MOTIF 57 67 "HIGH" region.
 MOTIF 406 410 "KMSKS" region.
 METAL 55 55 Zinc (By similarity).
 METAL 348 348 Zinc (By similarity).
 METAL 373 373 Zinc (By similarity).
 METAL 377 377 Zinc (By similarity).
 BINDING 409 409 ATP (By similarity).
 MOD_RES 307 307 Phosphoserine (By similarity).
 MOD_RES 503 503 N6-acetyllysine.
 MOD_RES 746 746 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Aminoacyl-tRNA synthetase; ATP-binding; Chromosomal rearrangement; Complete proteome; Cytoplasm; Ligase; Metal-binding; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Proto-oncogene; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 748 AA 
Protein Sequence
MADSSGQQAP DYRSILSISD EAARAQALNE HLSTRSYVQG YSLSQADVDA FRQLSAPPAD 60
PQLFHVARWF RHIEALLGSP CGKGQPCRLQ ASKGRRVQPQ WSPPAGTQPC RLHLYNSLTR 120
NKEVFIPQDG KKVTWYCCGP TVYDASHMGH ARSYISFDIL RRVLKDYFKF DVFYCMNITD 180
IDDKIIKRAR QNHLFEQYRE KRPEAAQLLE DVQAALKPFS VKLNETTDPD KKQMLERIQH 240
AVQLATEPLE KAVQSRLTGE EVNSCVEVLL EEAKDLLSDW LDSTLGCDVT DNSIFSKLPK 300
FWEGDFHRDM EALNVLPPDV LTRVSEYVPE IVNFVQKIVD NGYGYVSNGS VYFDTAKFAS 360
SEKHSYGKLV PEAVGDQKAL QEGEGDLSIS ADRLSEKRSP NDFALWKASK PGEPSWPCPW 420
GKGRPGWHIE CSAMAGTLLG ASMDIHGGGF DLRFPHHDNE LAQSEAYFEN DCWVRYFLHT 480
GHLTIAGCKM SKSLKNFITI KDALKKHSAR QLRLAFLMHS WKDTLDYSSN TMESALQYEK 540
FLNEFFLNVK DILRAPVDIT GQFEKWGEEE AELNKNFYDK KTAIHKALCD NVDTRTVMEE 600
MRALVSQCNL YMAARKAVRK RPNQALLENI ALYLTHMLKI FGAVEEDSSL GFPVGGPGTS 660
LSLEATVMPY LQVLSEFREG VRKIAREQKV PEILQLSDAL RDNILPELGV RFEDHEGLPT 720
VVKLVDRNTL LKEREEKRRV EEEKRKKKEE AARRKQEQEA AKLAKMKIPP SEMFLSETDK 780
YSKFDENGLP THDMEGKELS KGQAKKLKKL FEAQEKLYKE YLQMAQNGSF Q 831 
Gene Ontology
 GO:0005737; C:cytoplasm; NAS:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0004817; F:cysteine-tRNA ligase activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0000049; F:tRNA binding; IDA:UniProtKB.
 GO:0006423; P:cysteinyl-tRNA aminoacylation; IDA:UniProtKB. 
Interpro
 IPR015803; Cys-tRNA-ligase.
 IPR024909; Cys-tRNA/MSH_ligase.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd. 
Pfam
 PF01406; tRNA-synt_1e 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00983; TRNASYNTHCYS.