CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001667
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 BAG family molecular chaperone regulator 2 
Protein Synonyms/Alias
 BAG-2; Bcl-2-associated athanogene 2 
Gene Name
 BAG2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MAQAKINAKANEubiquitination[1, 2, 3]
9AQAKINAKANEGRFCubiquitination[4, 5]
111PQQQESLKHATRIIDacetylation[6]
111PQQQESLKHATRIIDubiquitination[2, 4, 5]
123IIDEVVNKFLDDLGNubiquitination[2, 4, 5]
154PHGPVDQKFQSIVIGubiquitination[2]
169CALEDQKKIKRRLETubiquitination[2]
186RNIENSDKAIKLLEHubiquitination[2, 4, 5]
189ENSDKAIKLLEHSKGubiquitination[2]
195IKLLEHSKGAGSKTLubiquitination[4, 5, 7]
200HSKGAGSKTLQQNAEubiquitination[1, 2, 4, 5, 7, 8, 9]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. 
Sequence Annotation
 DOMAIN 109 189 BAG.
 MOD_RES 20 20 Phosphoserine.
 MOD_RES 73 73 Phosphoserine.  
Keyword
 Chaperone; Coiled coil; Complete proteome; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 211 AA 
Protein Sequence
MAQAKINAKA NEGRFCRSSS MADRSSRLLE SLDQLELRVE ALREAATAVE QEKEILLEMI 60
HSIQNSQDMR QISDGEREEL NLTANRLMGR TLTVEVSVET IRNPQQQESL KHATRIIDEV 120
VNKFLDDLGN AKSHLMSLYS ACSSEVPHGP VDQKFQSIVI GCALEDQKKI KRRLETLLRN 180
IENSDKAIKL LEHSKGAGSK TLQQNAESRF N 211 
Gene Ontology
 GO:0006457; P:protein folding; TAS:ProtInc. 
Interpro
 IPR003103; BAG_domain. 
Pfam
 PF02179; BAG 
SMART
 SM00264; BAG 
PROSITE
 PS51035; BAG 
PRINTS