CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000981
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Importin subunit alpha-7 
Protein Synonyms/Alias
 Karyopherin subunit alpha-6 
Gene Name
 KPNA6 
Gene Synonyms/Alias
 IPOA7 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9ETMASPGKDNYRMKSubiquitination[1]
18NYRMKSYKNNALNPEubiquitination[1, 2, 3]
49KREQQLFKRRNVELIubiquitination[2, 3]
110KFRKLLSKEPSPPIDubiquitination[1, 3]
134DRFVEFLKRNENCTLubiquitination[1, 3, 4]
249NPPPEFAKVSPCLPVubiquitination[1]
354SPKESIRKEACWTISubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. 
Sequence Annotation
 DOMAIN 1 60 IBB.
 REPEAT 76 115 ARM 1; truncated.
 REPEAT 116 159 ARM 2.
 REPEAT 160 204 ARM 3.
 REPEAT 205 243 ARM 4.
 REPEAT 244 288 ARM 5.
 REPEAT 289 328 ARM 6.
 REPEAT 329 370 ARM 7.
 REPEAT 371 410 ARM 8.
 REPEAT 411 453 ARM 9.
 REPEAT 457 502 ARM 10; atypical.
 REGION 147 239 NLS binding site (major) (By similarity).
 REGION 316 404 NLS binding site (minor) (By similarity).
 MOTIF 45 54 Nuclear localization signal (By
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 6 6 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 536 AA 
Protein Sequence
METMASPGKD NYRMKSYKNN ALNPEEMRRR REEEGIQLRK QKREQQLFKR RNVELINEEA 60
AMFDSLLMDS YVSSTTGESV ITREMVEMLF SDDSDLQLAT TQKFRKLLSK EPSPPIDEVI 120
NTPRVVDRFV EFLKRNENCT LQFEAAWALT NIASGTSQQT KIVIEAGAVP IFIELLNSDF 180
EDVQEQAVWA LGNIAGDSSV CRDYVLNCSI LNPLLTLLTK STRLTMTRNA VWALSNLCRG 240
KNPPPEFAKV SPCLPVLSRL LFSSDSDLLA DACWALSYLS DGPNEKIQAV IDSGVCRRLV 300
ELLMHNDYKV ASPALRAVGN IVTGDDIQTQ VILNCSALPC LLHLLSSPKE SIRKEACWTI 360
SNITAGNRAQ IQAVIDANIF PVLIEILQKA EFRTRKEAAW AITNATSGGT PEQIRYLVSL 420
GCIKPLCDLL TVMDSKIVQV ALNGLENILR LGEQEGKRSG SGVNPYCGLI EEAYGLDKIE 480
FLQSHENQEI YQKAFDLIEH YFGVEDDDSS LAPQVDETQQ QFIFQQPEAP MEGFQL 536 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0008565; F:protein transporter activity; IEA:Compara.
 GO:0006607; P:NLS-bearing substrate import into nucleus; TAS:ProtInc. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR002652; Importin-a_IBB.
 IPR024931; Importing_su_alpha. 
Pfam
 PF00514; Arm
 PF01749; IBB 
SMART
 SM00185; ARM 
PROSITE
 PS50176; ARM_REPEAT
 PS51214; IBB 
PRINTS