CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009075
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Syntaxin-17 
Protein Synonyms/Alias
  
Gene Name
 STX17 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19RLEPAIQKFIKIVIPubiquitination[1]
22PAIQKFIKIVIPTDLubiquitination[1]
41KHQINIEKYQRCRIWacetylation[2]
273VLGFTGGKLIQRKKQubiquitination[3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 SNAREs, Soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi (PubMed:21545355). 
Sequence Annotation
 DOMAIN 162 224 t-SNARE coiled-coil homology.
 REGION 229 275 Necessary and sufficient for localization
 MOTIF 299 302 Endoplasmic reticulum retention signal.
 MOD_RES 41 41 N6-acetyllysine.
 MOD_RES 157 157 Phosphotyrosine; by ABL1.  
Keyword
 Acetylation; Autophagy; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Endoplasmic reticulum; ER-Golgi transport; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 302 AA 
Protein Sequence
MSEDEEKVKL RRLEPAIQKF IKIVIPTDLE RLRKHQINIE KYQRCRIWDK LHEEHINAGR 60
TVQQLRSNIR EIEKLCLKVR KDDLVLLKRM IDPVKEEASA ATAEFLQLHL ESVEELKKQF 120
NDEETLLQPP LTRSMTVGGA FHTTEAEASS QSLTQIYALP EIPQDQNAAE SWETLEADLI 180
ELSQLVTDFS LLVNSQQEKI DSIADHVNSA AVNVEEGTKN LGKAAKYKLA ALPVAGALIG 240
GMVGGPIGLL AGFKVAGIAA ALGGGVLGFT GGKLIQRKKQ KMMEKLTSSC PDLPSQTDKK 300
CS 302 
Gene Ontology
 GO:0000421; C:autophagic vacuole membrane; IDA:UniProtKB.
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IDA:UniProtKB.
 GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
 GO:0030134; C:ER to Golgi transport vesicle; ISS:UniProtKB.
 GO:0012507; C:ER to Golgi transport vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0030868; C:smooth endoplasmic reticulum membrane; ISS:UniProtKB.
 GO:0031201; C:SNARE complex; IDA:UniProtKB.
 GO:0005484; F:SNAP receptor activity; IMP:UniProtKB.
 GO:0000149; F:SNARE binding; IDA:UniProtKB.
 GO:0000046; P:autophagic vacuole fusion; IMP:UniProtKB.
 GO:0097111; P:endoplasmic reticulum-Golgi intermediate compartment organization; IMP:UniProtKB.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:UniProtKB.
 GO:0006887; P:exocytosis; IBA:RefGenome.
 GO:0007030; P:Golgi organization; IMP:UniProtKB.
 GO:0006886; P:intracellular protein transport; IBA:RefGenome. 
Interpro
 IPR006012; Syntaxin/epimorphin_CS.
 IPR010989; t-SNARE.
 IPR000727; T_SNARE_dom. 
Pfam
 PF05739; SNARE 
SMART
 SM00397; t_SNARE 
PROSITE
 PS00914; SYNTAXIN
 PS50192; T_SNARE 
PRINTS