CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032299
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein Wiz 
Protein Synonyms/Alias
 WIZ protein 
Gene Name
 WIZ 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
148PAVNKAIKSPPGFSAmethylation[1, 2]
426PSPKALAKMMGGAGPmethylation[3]
476SPPPTARKMFPGLAAmethylation[3]
911VGNIYTLKCRFCEVEubiquitination[4, 5, 6, 7, 8, 9]
944ILEMNFSKADPPPEEubiquitination[8]
Reference
 [1] A general molecular affinity strategy for global detection and proteomic analysis of lysine methylation.
 Moore KE, Carlson SM, Camp ND, Cheung P, James RG, Chua KF, Wolf-Yadlin A, Gozani O.
 Mol Cell. 2013 May 9;50(3):444-56. [PMID: 23583077]
 [2] Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome.
 Bremang M, Cuomo A, Agresta AM, Stugiewicz M, Spadotto V, Bonaldi T.
 Mol Biosyst. 2013 Jul 30;9(9):2231-47. [PMID: 23748837]
 [3] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 965 AA 
Protein Sequence
MDLGSPSLPK KSLPVPGALE QVASRLSSKV AAEVPHGSKQ ELQDLKAQSL TTCEVCGACF 60
ETRKGLSSHA RSHLRQLGVA ESESSGAPID LLYELVKQKG LPDAHLGLPP GLAKKSSSLK 120
EVVAGAPRPG LLSLAKPLDA PAVNKAIKSP PGFSAKGLGH PPSSPLLKKT PLALAGSPTP 180
KNPEDKSPQL SLSPRPASPK AQWPQSEDEG PLNLTLDSDG GRELDCQLCG AWFETRKGLS 240
SHARAHLRHL GVSDPDAKGS PIDVLHGLIR RDGVQIRLPP RRGALAHPGR PPPTSAALSL 300
LPPPPPAKKA KLKAAGMASP WGKQDLSAAA AAGIFWASDV EPSPLNLSSG PEPARDIRCE 360
FCGEFFENRK GLSSHARSHL RQMGVTEWYV NGSPIDTLRE ILKRRTQSRP GGPPNPPGPS 420
PKALAKMMGG AGPGSSLEAR SPSDLHISPL AKKLPPPPGS PLGHSPTASP PPTARKMFPG 480
LAAPSLPKKL KPEQIRVEIK REMLPGALHG ELHPSEGPWG APREDMTPLN LSSRAEPVRD 540
IRCEFCGEFF ENRKGLSSHA RSHLRQMGVT EWSVNGSPID TLREILKKKS KPCLIKKEPP 600
AGDLAPALAE DGPPTVAPGP VQSPLPLSPL AGRPGKPGAG PAQVPRELSL TPITGAKPSA 660
TGYLGSVAAK RPLQEDRLLP AEVKAKTYIQ TELPFKAKTL HEKTSHSSTE ACCELCGLYF 720
ENRKALASHA RAHLRQFGVT EWCVNGSPIE TLSEWIKHRP QKVGAYRSYI QGGRPFTKKF 780
RSAGHGRDSD KRPSLGLAPG GLAVVGRSAG GEPGPEAGRA ADGGERPLAA SPPGTVKAEE 840
HQRQNINKFE RRQARPPDAS AARGGEDTND LQQKLEEVRQ PPPRVRPVPS LVPRPPQTSL 900
VKFVGNIYTL KCRFCEVEFQ GPLSIQEEWV RHLQRHILEM NFSKADPPPE ESQAPQAQTA 960
AAEAP 965 
Gene Ontology
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS