UniProt Accession

 DCTN2_MOUSE; Q99KJ8 

Genbank Protein ID

 BC004613 

Genbank Nucleotide ID

 AAH04613.1 

Protein Name

 Dynactin subunit 2 

Protein Synonyms/Alias

 50 kDa dynein-associated polypeptide; Dynactin complex 50 kDa subunit; DCTN-50; Growth cone membrane protein 23-48K; GMP23-48K; p50 dynamitin 

Gene Name

 Dctn2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
132	KESATEEKLTPVVLA	ubiquitination	[1]
140	LTPVVLAKQLAALKQ	acetylation	[2, 3]
140	LTPVVLAKQLAALKQ	succinylation	[3]
140	LTPVVLAKQLAALKQ	ubiquitination	[1]
146	AKQLAALKQQLVASH	acetylation	[2]
175	DPDGALAKRLLLQLE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 6 6 Phosphotyrosine (By similarity).
 MOD_RES 83 83 Phosphoserine.
 MOD_RES 86 86 Phosphotyrosine.  

Keyword

 Acetylation; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Dynein; Membrane; Microtubule; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 402 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005869; C:dynactin complex; IEA:Compara.
 GO:0030286; C:dynein complex; IEA:UniProtKB-KW.
 GO:0030426; C:growth cone; IDA:MGI.
 GO:0000776; C:kinetochore; IEA:Compara.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0031982; C:vesicle; ISS:UniProtKB.
 GO:0003774; F:motor activity; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0007067; P:mitosis; IEA:Compara.
 GO:0007052; P:mitotic spindle organization; IEA:Compara. 

Interpro

 IPR006996; Dynamitin_2su. 

Pfam

  

SMART

  

PROSITE

  

PRINTS