CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002139
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calpain small subunit 1 
Protein Synonyms/Alias
 CSS1; Calcium-activated neutral proteinase small subunit; CANP small subunit; Calcium-dependent protease small subunit; CDPS; Calcium-dependent protease small subunit 1; Calpain regulatory subunit 
Gene Name
 CAPNS1 
Gene Synonyms/Alias
 CAPN4; CAPNS 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
135VTRHPDLKTDGFGIDubiquitination[1, 2]
158MDSDTTGKLGFEEFKubiquitination[1, 2]
172KYLWNNIKRWQAIYKubiquitination[1, 2, 3, 4]
179KRWQAIYKQFDTDRSacetylation[5]
179KRWQAIYKQFDTDRSubiquitination[1, 2, 3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861
Functional Description
 Regulatory subunit of the calcium-regulated non- lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. 
Sequence Annotation
 DOMAIN 91 125 EF-hand 1; atypical.
 DOMAIN 139 172 EF-hand 2.
 DOMAIN 169 204 EF-hand 3.
 DOMAIN 205 233 EF-hand 4.
 DOMAIN 234 268 EF-hand 5.
 METAL 109 109 Calcium 1; via carbonyl oxygen (By
 METAL 112 112 Calcium 1 (By similarity).
 METAL 114 114 Calcium 1; via carbonyl oxygen (By
 METAL 119 119 Calcium 1 (By similarity).
 METAL 137 137 Calcium 4 (By similarity).
 METAL 152 152 Calcium 2 (By similarity).
 METAL 154 154 Calcium 2 (By similarity).
 METAL 156 156 Calcium 2; via carbonyl oxygen (By
 METAL 158 158 Calcium 2; via carbonyl oxygen (By
 METAL 163 163 Calcium 2 (By similarity).
 METAL 182 182 Calcium 3 (By similarity).
 METAL 184 184 Calcium 3 (By similarity).
 METAL 186 186 Calcium 3; via carbonyl oxygen (By
 METAL 188 188 Calcium 3; via carbonyl oxygen (By
 METAL 193 193 Calcium 3 (By similarity).
 METAL 225 225 Calcium 4 (By similarity).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 179 179 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Calcium; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Membrane; Metal-binding; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 268 AA 
Protein Sequence
MFLVNSFLKG GGGGGGGGGG LGGGLGNVLG GLISGAGGGG GGGGGGGGGG GGGGGGTAMR 60
ILGGVISAIS EAAAQYNPEP PPPRTHYSNI EANESEEVRQ FRRLFAQLAG DDMEVSATEL 120
MNILNKVVTR HPDLKTDGFG IDTCRSMVAV MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ 180
FDTDRSGTIC SSELPGAFEA AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF 240
RAFKSLDKDG TGQIQVNIQE WLQLTMYS 268 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; TAS:ProtInc.
 GO:0008284; P:positive regulation of cell proliferation; TAS:ProtInc. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom. 
Pfam
 PF13405; EF_hand_4 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 
PRINTS