CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001493
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pre-mRNA-processing factor 6 
Protein Synonyms/Alias
 Androgen receptor N-terminal domain-transactivating protein 1; ANT-1; PRP6 homolog; U5 snRNP-associated 102 kDa protein; U5-102 kDa protein 
Gene Name
 PRPF6 
Gene Synonyms/Alias
 C20orf14 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
178QRNPRYEKLTPVPDSubiquitination[1, 2, 3, 4, 5]
189VPDSFFAKHLQTGENubiquitination[3]
324RLEEVTGKLQVARNLubiquitination[3]
408PNSVRLWKAAVELEEubiquitination[3, 5, 6]
472HIWITAAKLEEANGNubiquitination[3, 7]
485GNTQMVEKIIDRAITubiquitination[3]
574LQVFPSKKSVWLRAAubiquitination[3]
585LRAAYFEKNHGTRESacetylation[8]
585LRAAYFEKNHGTRESubiquitination[3, 5]
616VLWLMGAKSKWLAGDubiquitination[1, 4, 7, 9]
618WLMGAKSKWLAGDVPubiquitination[1, 3, 4]
680PTARVFMKSVKLEWVubiquitination[3]
710RHYEDFPKLWMMKGQubiquitination[3]
715FPKLWMMKGQIEEQKubiquitination[3, 6]
722KGQIEEQKEMMEKARubiquitination[3]
727EQKEMMEKAREAYNQubiquitination[3]
737EAYNQGLKKCPHSTPubiquitination[3]
738AYNQGLKKCPHSTPLubiquitination[3]
755LLSRLEEKIGQLTRAubiquitination[3]
768RARAILEKSRLKNPKubiquitination[3]
775KSRLKNPKNPGLWLEubiquitination[1, 3, 4]
793LEYRAGLKNIANTLMubiquitination[3]
829ARPQRRTKSVDALKKacetylation[5]
917ELWCAVSKDIANWQKubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Involved in pre-mRNA splicing as component of the U4/U6- U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation. 
Sequence Annotation
 REPEAT 384 416 HAT 1.
 REPEAT 418 444 HAT 2.
 REPEAT 445 476 HAT 3.
 REPEAT 554 586 HAT 4.
 REPEAT 588 620 HAT 5.
 REPEAT 622 654 HAT 6.
 REPEAT 689 721 HAT 7.
 REPEAT 723 755 HAT 8.
 REPEAT 855 887 HAT 9.
 MOD_RES 266 266 Phosphothreonine.
 MOD_RES 275 275 Phosphothreonine.
 MOD_RES 279 279 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Retinitis pigmentosa; Spliceosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 941 AA 
Protein Sequence
MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP PGKRTVGDQM 60
KKNQAADDDD EDLNDTNYDE FNGYAGSLFS SGPYEKDDEE ADAIYAALDK RMDERRKERR 120
EQREKEEIEK YRMERPKIQQ QFSDLKRKLA EVTEEEWLSI PEVGDARNKR QRNPRYEKLT 180
PVPDSFFAKH LQTGENHTSV DPRQTQFGGL NTPYPGGLNT PYPGGMTPGL MTPGTGELDM 240
RKIGQARNTL MDMRLSQVSD SVSGQTVVDP KGYLTDLNSM IPTHGGDIND IKKARLLLKS 300
VRETNPHHPP AWIASARLEE VTGKLQVARN LIMKGTEMCP KSEDVWLEAA RLQPGDTAKA 360
VVAQAVRHLP QSVRIYIRAA ELETDIRAKK RVLRKALEHV PNSVRLWKAA VELEEPEDAR 420
IMLSRAVECC PTSVELWLAL ARLETYENAR KVLNKARENI PTDRHIWITA AKLEEANGNT 480
QMVEKIIDRA ITSLRANGVE INREQWIQDA EECDRAGSVA TCQAVMRAVI GIGIEEEDRK 540
HTWMEDADSC VAHNALECAR AIYAYALQVF PSKKSVWLRA AYFEKNHGTR ESLEALLQRA 600
VAHCPKAEVL WLMGAKSKWL AGDVPAARSI LALAFQANPN SEEIWLAAVK LESENDEYER 660
ARRLLAKARS SAPTARVFMK SVKLEWVQDN IRAAQDLCEE ALRHYEDFPK LWMMKGQIEE 720
QKEMMEKARE AYNQGLKKCP HSTPLWLLLS RLEEKIGQLT RARAILEKSR LKNPKNPGLW 780
LESVRLEYRA GLKNIANTLM AKALQECPNS GILWSEAIFL EARPQRRTKS VDALKKCEHD 840
PHVLLAVAKL FWSQRKITKA REWFHRTVKI DSDLGDAWAF FYKFELQHGT EEQQEEVRKR 900
CESAEPRHGE LWCAVSKDIA NWQKKIGDIL RLVAGRIKNT F 941 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0071001; C:U4/U6 snRNP; IDA:BHF-UCL.
 GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IDA:BHF-UCL.
 GO:0005682; C:U5 snRNP; IDA:BHF-UCL.
 GO:0043021; F:ribonucleoprotein complex binding; IDA:BHF-UCL.
 GO:0003723; F:RNA binding; IDA:MGI.
 GO:0003713; F:transcription coactivator activity; IGI:MGI.
 GO:0000244; P:assembly of spliceosomal tri-snRNP; IMP:BHF-UCL.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0006403; P:RNA localization; IMP:MGI.
 GO:0000245; P:spliceosomal complex assembly; NAS:UniProtKB. 
Interpro
 IPR003107; HAT.
 IPR010491; PRP1_N.
 IPR027108; Prp6/Prp1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF06424; PRP1_N
 PF13181; TPR_8 
SMART
 SM00386; HAT 
PROSITE
  
PRINTS