CPLM-000721

Genbank Nucleotide ID

DNA repair protein RAD51 homolog 3

Protein Synonyms/Alias

R51H3; RAD51 homolog C; RAD51-like protein 2

Homo sapiens (Human)

Position	Peptide	Type	References
26	LSPAVRVKLVSAGFQ	ubiquitination	[1]
42	AEELLEVKPSELSKE	ubiquitination	[1, 2, 3]
48	VKPSELSKEVGISKA	ubiquitination	[1]
54	SKEVGISKAEALETL	ubiquitination	[1, 2, 3]
72	RRECLTNKPRYAGTS	ubiquitination	[2]
83	AGTSESHKKCTALEL	ubiquitination	[1]
328	QRLATLYKSPSQKEC	ubiquitination	[1]
342	CTVLFQIKPQGFRDT	ubiquitination	[2]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The RAD51B-RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Participates in branch migration and Holliday junction resolution and thus is important for processing HR intermediates late in the DNA repair process. Also has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHEK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis.

NP_BIND 125 132 ATP (Potential).
MOTIF 366 370 Nuclear localization signal (Potential).

Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Disease mutation; DNA damage; DNA recombination; DNA repair; DNA-binding; Fanconi anemia; Mitochondrion; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005739; C:mitochondrion; IDA:UniProtKB.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:Compara.
GO:0003677; F:DNA binding; TAS:ProtInc.
GO:0008094; F:DNA-dependent ATPase activity; IEA:InterPro.
GO:0007596; P:blood coagulation; TAS:Reactome.
GO:0006310; P:DNA recombination; IDA:UniProtKB.
GO:0006281; P:DNA repair; IDA:UniProtKB.
GO:0007066; P:female meiosis sister chromatid cohesion; IEA:Compara.
GO:0007141; P:male meiosis I; IEA:Compara.
GO:0007131; P:reciprocal meiotic recombination; IEA:Compara.
GO:0007062; P:sister chromatid cohesion; ISS:UniProtKB.
GO:0007283; P:spermatogenesis; IEA:Compara.

IPR003593; AAA+_ATPase.
IPR013632; DNA_recomb/repair_Rad51_C.
IPR016467; DNA_recomb/repair_RecA-like.
IPR020588; DNA_recomb_RecA/RadB_ATP-bd.
IPR027417; P-loop_NTPase.