CPLM-004768

Genbank Nucleotide ID

DNA-directed RNA polymerases I, II, and III subunit RPABC1

Protein Synonyms/Alias

RNA polymerases I, II, and III subunit ABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4

Homo sapiens (Human)

Position	Peptide	Type	References
15	YRLWKIRKTIMQLCH	ubiquitination	[1]
85	EEPKVGIKTIKVYCQ	ubiquitination	[2]
88	KVGIKTIKVYCQRMQ	ubiquitination	[1, 3]
115	QGMTPSAKQSLVDMA	ubiquitination	[1, 4, 5, 6, 7]
186	VARYFGIKRGQVVKI	ubiquitination	[1]
192	IKRGQVVKIIRPSET	ubiquitination	[1, 2, 4, 5]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).

MOD_RES 1 1 N-acetylmethionine.

Acetylation; Complete proteome; Direct protein sequencing; DNA-directed RNA polymerase; Host-virus interaction; Nucleus; Polymorphism; Reference proteome; Transcription.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005665; C:DNA-directed RNA polymerase II, core complex; IDA:UniProtKB.
GO:0003677; F:DNA binding; IEA:InterPro.
GO:0003899; F:DNA-directed RNA polymerase activity; TAS:ProtInc.
GO:0006370; P:7-methylguanosine mRNA capping; TAS:Reactome.
GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
GO:0006386; P:termination of RNA polymerase III transcription; TAS:Reactome.
GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
GO:0006385; P:transcription elongation from RNA polymerase III promoter; TAS:Reactome.
GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome.
GO:0022415; P:viral reproductive process; TAS:Reactome.
GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.

IPR014381; DNA_RNA_pol_RPB5_euk/virus.
IPR005571; RNA_pol_Rpb5_N.
IPR000783; RNA_pol_subH/Rpb5_C.
IPR020608; RNA_pol_subH/Rpb5_CS.
IPR020609; RpoH/RPB5.

PF01191; RNA_pol_Rpb5_C
PF03871; RNA_pol_Rpb5_N

PS01110; RNA_POL_H_23KD