CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002586
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 MAP kinase kinase PBS2 
Protein Synonyms/Alias
 Polymyxin B resistance protein 2; Suppressor of fluoride sensitivity 4 
Gene Name
 PBS2 
Gene Synonyms/Alias
 HOG4; SFS4; SSK4; YJL128C; J0699 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
487NIIHRDVKPTNILCSubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Seems to phosphorylate HOG1 on a tyrosine residue. 
Sequence Annotation
 DOMAIN 360 623 Protein kinase.
 NP_BIND 366 374 ATP (By similarity).
 ACT_SITE 485 485 Proton acceptor (By similarity).
 BINDING 389 389 ATP (By similarity).
 MOD_RES 269 269 Phosphoserine.
 MOD_RES 514 514 Phosphoserine.
 MOD_RES 518 518 Phosphothreonine.  
Keyword
 3D-structure; Antibiotic resistance; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 668 AA 
Protein Sequence
MEDKFANLSL HEKTGKSSIQ LNEQTGSDNG SAVKRTSSTS SHYNNINADL HARVKAFQEQ 60
RALKRSASVG SNQSEQDKGS SQSPKHIQQI VNKPLPPLPV AGSSKVSQRM SSQVVQASSK 120
STLKNVLDNQ ETQNITDVNI NIDTTKITAT TIGVNTGLPA TDITPSVSNT ASATHKAQLL 180
NPNRRAPRRP LSTQHPTRPN VAPHKAPAII NTPKQSLSAR RGLKLPPGGM SLKMPTKTAQ 240
QPQQFAPSPS NKKHIETLSN SKVVEGKRSN PGSLINGVQS TSTSSSTEGP HDTVGTTPRT 300
GNSNNSSNSG SSGGGGLFAN FSKYVDIKSG SLNFAGKLSL SSKGIDFSNG SSSRITLDEL 360
EFLDELGHGN YGNVSKVLHK PTNVIMATKE VRLELDEAKF RQILMELEVL HKCNSPYIVD 420
FYGAFFIEGA VYMCMEYMDG GSLDKIYDES SEIGGIDEPQ LAFIANAVIH GLKELKEQHN 480
IIHRDVKPTN ILCSANQGTV KLCDFGVSGN LVASLAKTNI GCQSYMAPER IKSLNPDRAT 540
YTVQSDIWSL GLSILEMALG RYPYPPETYD NIFSQLSAIV DGPPPRLPSD KFSSDAQDFV 600
SLCLQKIPER RPTYAALTEH PWLVKYRNQD VHMSEYITER LERRNKILRE RGENGLSKNV 660
PALHMGGL 668 
Gene Ontology
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0031416; C:NatB complex; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004708; F:MAP kinase kinase activity; IMP:SGD.
 GO:0004596; F:peptide alpha-N-acetyltransferase activity; IMP:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
 GO:0007015; P:actin filament organization; IMP:SGD.
 GO:0000169; P:activation of MAPK activity involved in osmosensory signaling pathway; IMP:SGD.
 GO:0034605; P:cellular response to heat; IMP:SGD.
 GO:0006972; P:hyperosmotic response; IMP:SGD.
 GO:0000208; P:MAPK import into nucleus involved in osmosensory signaling pathway; IMP:SGD.
 GO:0017196; P:N-terminal peptidyl-methionine acetylation; IGI:UniProtKB.
 GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS