CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008934
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Developmentally-regulated GTP-binding protein 2 
Protein Synonyms/Alias
 DRG-2 
Gene Name
 DRG2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
46AQLLEPSKSASSKGEubiquitination[1]
51PSKSASSKGEGFDVMubiquitination[1, 2]
59GEGFDVMKSGDARVAubiquitination[1, 2]
127IEGAAQGKGRGRQVIubiquitination[1, 2, 3, 4, 5]
151IMMLDATKGEVQRSLubiquitination[1, 2]
161VQRSLLEKELESVGIubiquitination[1, 2, 5, 6]
341LVWGTSTKYSPQRVGubiquitination[2, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 May play a role in cell proliferation, differentiation and death. 
Sequence Annotation
 DOMAIN 74 172 G.
 NP_BIND 69 76 GTP (By similarity).
 NP_BIND 115 119 GTP (By similarity).
 NP_BIND 246 249 GTP (By similarity).
 MOD_RES 199 199 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Cytoplasm; GTP-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 364 AA 
Protein Sequence
MGILEKISEI EKEIARTQKN KATEYHLGLL KAKLAKYRAQ LLEPSKSASS KGEGFDVMKS 60
GDARVALIGF PSVGKSTFLS LMTSTASEAA SYEFTTLTCI PGVIEYKGAN IQLLDLPGII 120
EGAAQGKGRG RQVIAVARTA DVIIMMLDAT KGEVQRSLLE KELESVGIRL NKHKPNIYFK 180
PKKGGGISFN STVTLTQCSE KLVQLILHEY KIFNAEVLFR EDCSPDEFID VIVGNRVYMP 240
CLYVYNKIDQ ISMEEVDRLA RKPNSVVISC GMKLNLDYLL EMLWEYLALT CIYTKKRGQR 300
PDFTDAIILR KGASVEHVCH RIHRSLASQF KYALVWGTST KYSPQRVGLT HTMEHEDVIQ 360
IVKK 364 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005525; F:GTP binding; TAS:ProtInc.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR006074; GTP1-OBG_CS.
 IPR006073; GTP_binding_domain.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR004095; TGS.
 IPR012676; TGS-like. 
Pfam
 PF01926; MMR_HSR1
 PF02824; TGS 
SMART
  
PROSITE
 PS00905; GTP1_OBG 
PRINTS
 PR00326; GTP1OBG.