CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005472
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Siroheme synthase 
Protein Synonyms/Alias
 Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase 
Gene Name
 cysG 
Gene Synonyms/Alias
 STM3477 
Created Date
 July 27, 2013 
Organism
 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
NCBI Taxa ID
 99287 
Lysine Modification
Position
Peptide
Type
References
292ILLREAQKGKRVVRLacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD- dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme. 
Sequence Annotation
 REGION 218 457 Uroporphyrinogen-III C-methyltransferase.  
Keyword
 3D-structure; Cobalamin biosynthesis; Complete proteome; Lyase; Methyltransferase; Multifunctional enzyme; NAD; Oxidoreductase; Porphyrin biosynthesis; Reference proteome; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 457 AA 
Protein Sequence
MDHLPIFCQL RDRDCLIVGG GDVAERKARL LLEAGARLTV NALTFIPQFT VWANEGMLTL 60
VEGPFDETLL DSCWLAIAAT DDDTVNQRVS DAAESRRIFC NVVDAPKAAS FIMPSIIDRS 120
PLMVAVSSGG TSPVLARLLR EKLESLLPQH LGQVARYAGQ LRARVKKQFA TMGERRRFWE 180
KFFVNDRLAQ SLANADEKAV NATTERLFSE PLDHRGEVVL VGAGPGDAGL LTLKGLQQIQ 240
QADIVVYDRL VSDDIMNLVR RDADRVFVGK RAGYHCVPQE EINQILLREA QKGKRVVRLK 300
GGDPFIFGRG GEELETLCHA GIPFSVVPGI TAASGCSAYS GIPLTHRDYA QSVRLVTGHL 360
KTGGELDWEN LAAEKQTLVF YMGLNQAATI QEKLIAFGMQ ADMPVALVEN GTSVKQRVVH 420
GVLTQLGELA QQVESPALII VGRVVALRDK LNWFSNH 457 
Gene Ontology
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:HAMAP.
 GO:0051266; F:sirohydrochlorin ferrochelatase activity; IEA:EC.
 GO:0004851; F:uroporphyrin-III C-methyltransferase activity; IEA:HAMAP.
 GO:0009236; P:cobalamin biosynthetic process; IEA:HAMAP.
 GO:0019354; P:siroheme biosynthetic process; IEA:HAMAP. 
Interpro
 IPR000878; 4pyrrol_Mease.
 IPR014777; 4pyrrole_Mease_sub1.
 IPR014776; 4pyrrole_Mease_sub2.
 IPR006366; CobA/CysG_C.
 IPR016040; NAD(P)-bd_dom.
 IPR012409; Sirohaem_synth.
 IPR019478; Sirohaem_synthase_dimer_dom.
 IPR006367; Sirohaem_synthase_N.
 IPR003043; Uropor_MeTrfase_CS. 
Pfam
 PF10414; CysG_dimeriser
 PF13241; NAD_binding_7
 PF00590; TP_methylase 
SMART
  
PROSITE
 PS00839; SUMT_1
 PS00840; SUMT_2 
PRINTS