CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023780
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peptidyl-tRNA hydrolase 2, mitochondrial 
Protein Synonyms/Alias
 PTH 2; Bcl-2 inhibitor of transcription 1 
Gene Name
 PTRH2 
Gene Synonyms/Alias
 BIT1; PTH2; CGI-147 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
47LPKSKTSKTHTDTESubiquitination[1, 2, 3, 4, 5]
76LVVRNDLKMGKGKVAubiquitination[2, 4]
79RNDLKMGKGKVAAQCubiquitination[2]
95HAAVSAYKQIQRRNPubiquitination[1, 3, 5]
106RRNPEMLKQWEYCGQubiquitination[3, 4]
119GQPKVVVKAPDEETLubiquitination[2, 4]
134IALLAHAKMLGLTVSubiquitination[2, 4]
171GPADLIDKVTGHLKLubiquitination[2, 3, 4]
177DKVTGHLKLY*****ubiquitination[1, 2, 3, 4, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis (By similarity). 
Sequence Annotation
  
Keyword
 3D-structure; Apoptosis; Complete proteome; Hydrolase; Mitochondrion; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 179 AA 
Protein Sequence
MPSKSLVMEY LAHPSTLGLA VGVACGMCLG WSLRVCFGML PKSKTSKTHT DTESEASILG 60
DSGEYKMILV VRNDLKMGKG KVAAQCSHAA VSAYKQIQRR NPEMLKQWEY CGQPKVVVKA 120
PDEETLIALL AHAKMLGLTV SLIQDAGRTQ IAPGSQTVLG IGPGPADLID KVTGHLKLY 179 
Gene Ontology
 GO:0005829; C:cytosol; IMP:UniProtKB.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0004045; F:aminoacyl-tRNA hydrolase activity; IEA:EC.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:2000811; P:negative regulation of anoikis; IMP:UniProtKB.
 GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
 GO:2000210; P:positive regulation of anoikis; IMP:UniProtKB. 
Interpro
 IPR023476; Pep_tRNA_hydro_II_dom.
 IPR002833; Pep_tRNA_hydro_PTH2. 
Pfam
 PF01981; PTH2 
SMART
  
PROSITE
  
PRINTS